B‐Cell Progenitors and Precursors Change Their Microenvironment in Fetal Liver During Early Development
B‐Cell Progenitors and Precursors Change Their Microenvironment in Fetal Liver During Early Development
复制标题
BâCell 祖细胞和前体在早期发育过程中改变胎儿肝脏中的微环境
作者：
Tsuneto M;Tokoyoda K;Kajikhina E;Hauser AE;Hara T;Tani-Ichi S;Ikuta K;Melchers F
胎儿肝脏中B淋巴细胞发育的微环境在很大程度上仍然是未知的。在非造血细胞中，我们已经鉴定并FACS分离了两个亚群，即CD 45 − TER 119 −VCAM-1+细胞，它们要么是CD 105 highLYVE-1high，要么是CD 105 lowALCAM high。免疫组织化学分析发现，在胚胎第13.5天，4个c-Kit+IL-7 R α+ B220 lowCD 19 −SLC−B祖细胞中有3个与血管内皮型LYVE-1 high细胞接触。一天后，c-Kit+IL-7 R α+细胞发育为CD 19 −和+、SLC表达、DHJH重排的pre/pro和pro/preB-I细胞。不到10%的人仍与LYVE-1高细胞接触，但其中一半人现在与间充质来源的ALCAM高肝细胞接触。所有这些ALCAM高表达细胞，而不是LYVE-1高表达细胞，都能产生IL-7和CXCL 12，而两者都能产生CXCL 10。在体外，祖细胞和pro/preB-I细胞被趋化因子趋化至CXCL 10和12，这表明具有种系构型的IG基因位点的淋巴祖细胞在E13.5从血管内皮进入发育中的胎肝，被CXCL 10吸引，然后在一天内迁移至ALCAM高肝细胞微环境，分化为DHJH重排、替代轻链表达的pre/proB和pro/preB-I细胞，被CXCL 10和12吸引。在E15.5和E16.5之间，preB-I细胞在与ALCAM high细胞持续接触时扩增10倍，并在进一步分化的c-Kit−IL-7 R α−preBII细胞中开始VH-至DHJH-重排。干细胞2013;31：2800-2812
The microenvironments, in which B lymphocytes develop in fetal liver, are largely still unknown. Among the nonhematopoietic cells, we have identified and FACS-separated two subpopulations, CD45−TER119−VCAM-1+cells that are either CD105highLYVE-1highor CD105lowALCAMhigh. Immunohistochemical analyses find three of four c-Kit+IL-7Rα+B220lowCD19−SLC−B progenitors in contact with vascular endothelial-type LYVE-1highcells on embryonic day 13.5. One day later c-Kit+IL-7Rα+cells develop to CD19− and +, SLC-expressing, DHJH-rearranged pre/pro and pro/preB-I cells. Less than 10% are still in contact with LYVE-1highcells, but half of them are now in contact with mesenchymally derived ALCAMhighliver cells. All of these ALCAMhighcells, but not the LYVE-1highcells produce IL-7 and CXCL12, while both produce CXCL10. Progenitors and pro/preB-I cells are chemoattracted in vitro toward CXCL10 and 12, suggesting that lymphoid progenitors with Ig gene loci in germline configuration enter the developing fetal liver at E13.5 from vascular endothelium, attracted by CXCL10, and then migrate within a day to an ALCAMhighliver cell microenvironment, differentiating to DHJH-rearranging, surrogate light chain-expressing pre/proB and pro/preB-I cells, attracted by CXCL10 and 12. Between E15.5 and E16.5 preB-I cells expand 10-fold in continued contact with ALCAMhighcells and begin VH- to DHJH-rearrangements in further differentiated c-Kit−IL-7Rα−preBII cells. STEMCells2013;31:2800–2812
登录
查看更多内容
DOI：
10.1073/pnas.95.16.9448
发表时间：
1998-08-04
影响因子：
11.1
作者：
Ma, Q;Jones, D;Springer, TA
通讯作者：
Springer, TA
影响因子：
4.4
作者：
Hara, Takahiro;Shitara, Soichiro;Ikuta, Koichi
通讯作者：
Ikuta, Koichi
DOI：
10.1073/pnas.92.3.773
发表时间：
1995-01-31
影响因子：
11.1
作者：
GODIN, I;DIETERLENLIEVRE, F;CUMANO, A
通讯作者：
CUMANO, A
影响因子：
5.4
作者：
NISHIKAWA, SI;OGAWA, M;KODAMA, H
通讯作者：
KODAMA, H
影响因子：
4.4
作者：
P. Welch;P. Burrows;A. Namen;S. Gillis;M. Cooper
通讯作者：
P. Welch;P. Burrows;A. Namen;S. Gillis;M. Cooper