Dopamine agonist signalling in the hypothalamus of female rats is independent of calcium-dependent kinases.
Dopamine agonist signalling in the hypothalamus of female rats is independent of calcium-dependent kinases.
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DOI：
10.1111/j.1365-2826.2009.01917.x
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发表时间：
2009-11
影响因子：
3.2
通讯作者：
Mani SK
中科院分区：
文献类型：
--
作者：
Balasubramanian B;Mani SK
We have previously has demonstrated that dopamine agonist, SKF38396 (SKF), can substitute for progesterone (P) in the facilitation of female reproductive behavior in oestradiol benzoate (EB)-primed female rats and mice. We also reported that both P- and SKF-initiated signalling was mediated by cAMP-dependent PKA signal transduction cascade. As the rapid effects of P are also mediated by calcium-dependent kinases, calcium and calmodulin-dependent kinase (CaMKII) and protein kinase (PKC), we sought to determine whether SKF-initiated signalling also recruited calcium as a second messenger. We measured the changes in the activation of CaMKII and PKCin the ventromedial nucleus (VMN) of the hypothalamus and preoptic area (POA) of the rat brain, the two regions implicated in the regulation of female reproductive behavior in rodents. We measured the basal activities representing the activation of the kinases by in vivo treatments as well as the total kinase activities assayed in the presence of exogenous cofactors in vitro. We report that in contrast to P-initiated signalling, there was no recruitment of calcium by SKF in the hypothalamus as shown by the absence of changes in CaMKII activities in the VMN and POA. Furthermore, SKF-treatment resulted in a rapid increase in calcium-independent basal PKC activity in the VMN but not the POA. These rapid changes were not due to changes in PKC protein levels or phosphorylation status. These data indicate that P and SKF-recruit distinct signalling molecules within the same regions of the brain to activate region-specific signal transduction pathways.
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影响因子：
3.5
作者：
González-Flores, O;Ramírez-Orduña, JM;Beyer, C
通讯作者：
Beyer, C
影响因子：
56.9
作者：
Mani, SK;Fienberg, AA;O'Malley, BW
通讯作者：
O'Malley, BW
DOI：
10.1016/j.molbrainres.2004.05.009
发表时间：
2004-08-23
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Oster, H;Eichele, G;Leitges, M
通讯作者：
Leitges, M
影响因子：
4.8
作者：
Hernandez, AI;Blace, N;Sacktor, TC
通讯作者：
Sacktor, TC
影响因子：
4.8
作者：
Dewing, Phoebe;Christensen, Amy;Micevych, Paul
通讯作者：
Micevych, Paul