The common YAP activation mediates corneal epithelial regeneration and repair with different-sized wounds.
The common YAP activation mediates corneal epithelial regeneration and repair with different-sized wounds.
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常见的YAP激活介导不同大小伤口的角膜上皮再生和修复
DOI：
10.1038/s41536-021-00126-2
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发表时间：
2021-03-26
影响因子：
7.2
通讯作者：
Xu H
中科院分区：
文献类型：
--
作者：
Li Y;Ge L;Chen X;Mao Y;Gu X;Ren B;Zeng Y;Chen M;Chen S;Liu J;Yang Y;Xu H
损伤后的再生/修复可以由成体干细胞（ASC）或谱系限制的甚至终末分化的细胞赋予。在角膜上皮中，大伤口后的再生依赖于ASC（角膜缘上皮干细胞，LESC），而小伤口后的修复则不依赖于LESC。在这里，使用不同大小的大鼠角膜上皮伤口，我们表明，雅普激活促进大伤口后LESC的激活和扩增，以及小伤口后局部上皮细胞（修复上皮细胞）的重编程，这分别有助于LESC依赖性和非依赖性伤口愈合。在机械方面，我们强调了雅普活性的相互调节以及细胞连接和皮质F-肌动蛋白细胞骨架的组装加速了不同大小伤口的角膜上皮愈合。共同的雅普激活和潜在的调节机制被LESC和谱系限制的上皮细胞利用，以科普不同大小的角膜上皮伤口。
Regeneration/repair after injury can be endowed by adult stem cells (ASCs) or lineage restricted and even terminally differentiated cells. In corneal epithelium, regeneration after a large wound depends on ASCs (limbal epithelial stem cells, LESCs), whereas repair after a small wound is LESCs-independent. Here, using rat corneal epithelial wounds with different sizes, we show that YAP activation promotes the activation and expansion of LESCs after a large wound, as well as the reprogramming of local epithelial cells (repairing epithelial cells) after a small wound, which contributes to LESCs-dependent and -independent wound healing, respectively. Mechanically, we highlight that the reciprocal regulation of YAP activity and the assembly of cell junction and cortical F-actin cytoskeleton accelerates corneal epithelial healing with different-sized wounds. Together, the common YAP activation and the underlying regulatory mechanism are harnessed by LESCs and lineage-restricted epithelial cells to cope with corneal epithelial wounds with different sizes.
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影响因子：
23.9
作者：
Jadhav U;Saxena M;O'Neill NK;Saadatpour A;Yuan GC;Herbert Z;Murata K;Shivdasani RA
通讯作者：
Shivdasani RA
影响因子：
7.5
作者：
Li Y;Yang Y;Yang L;Zeng Y;Gao X;Xu H
通讯作者：
Xu H
影响因子：
4.6
作者：
Kasetti RB;Gaddipati S;Tian S;Xue L;Kao WW;Lu Q;Li Q
通讯作者：
Li Q
影响因子：
1.2
作者：
Dorà NJ;Hill RE;Collinson JM;West JD
通讯作者：
West JD
影响因子：
4
作者：
Kanellos, Georgios;Frame, Margaret C.
通讯作者：
Frame, Margaret C.