Long Period Grating Mach-Zehnder Interferometer Based Immunosensor with Temperature and Bulk Refractive Index Compensation.
Long Period Grating Mach-Zehnder Interferometer Based Immunosensor with Temperature and Bulk Refractive Index Compensation.
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DOI：
10.3390/bios12121099
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发表时间：
2022-11-30
期刊：
影响因子：
5.4
通讯作者：
Korposh, Serhiy
中科院分区：
文献类型：
--
作者：
Wu, Peizhou;Liu, Liangliang;Morgan, Stephen P.;Correia, Ricardo;Korposh, Serhiy
研制了一种用于免疫球蛋白M（IgM）检测的长周期光栅马赫-曾德尔干涉仪（LPGMZI）。由于纤芯模和包层模之间的干涉，测量的光谱具有条纹。该免疫传感器继承了LPG的优点，并且具有通过分析干涉条纹及其包络来补偿由于体折射率（RI）和温度波动引起的不需要的信号变化的潜力。在两种不同的情况下测量的外部RI从1.3384到1.3670：（i）仅两个LPG之间的连接部分被浸没或（ii）整个LPGMZI被浸没。在这两种情况下，条纹随着外部RI而移动，而包络在情况（i）中保持静止，或者在情况（ii）中以与条纹相同的速率移动。还通过将整个LPGMZI置于水浴中在25 °C和30 °C之间的不同温度下表征LPGMZI。条纹和包络线随温度以相同的速率移动。然后使用LPGMZI平台来创建IgM免疫传感器。两个LPG之间的连接部分用抗IgM功能化，并浸入IgM浓度为20 μg/mL至320 μg/mL的溶液中。条纹随着IgM浓度而移动，并且包膜保持静止。这项工作的结果表明，LPGMZI有可能补偿温度和散装RI波动，并作为一个便携式生物传感器平台。
A long period grating Mach–Zehnder interferometer (LPGMZI) that consists of two identical long period gratings (LPGs) in a single fibre was developed to measure immunoglobulin M (IgM). The measured spectrum has fringes due to the interference between the core mode and cladding mode. This immunosensor inherits the advantages of an LPG and has the potential to compensate for unwanted signal changes due to bulk refractive index (RI) and temperature fluctuations by analysing interference fringes and their envelope. The external RI was measured from 1.3384 to 1.3670 in two different cases: (i) only the connecting section between the two LPGs is immersed or (ii) the whole LPGMZI is immersed. The fringes shift with an external RI in both scenarios, whereas the envelope stays still in case (i) or shifts at the same rate as the fringes in case (ii). The LPGMZI was also characterised at different temperatures between 25 °C and 30 °C by placing the whole LPGMZI in a water bath. The fringes and envelope shift at the same rate with temperature. The LPGMZI platform was then used to create an IgM immunosensor. The connecting section between the two LPGs was functionalised with anti-IgM and immersed into solutions with IgM concentrations from 20 μg/mL to 320 μg/mL. The fringes shift with IgM concentration and the envelope remains static. The results from this work show that LPGMZI has the potential to compensate for the temperature and bulk RI fluctuations and perform as a portable biosensor platform.
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