Nanoplasmonic Structure of a Polycarbonate Substrate Integrated with Parallel Microchannels for Label-Free Multiplex Detection.
Nanoplasmonic Structure of a Polycarbonate Substrate Integrated with Parallel Microchannels for Label-Free Multiplex Detection.
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与平行微通道集成的聚碳酸酯基底的纳米等离子体结构，用于无标记多重检测。
DOI：
10.3390/polym13193294
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发表时间：
2021-09-27
期刊：
影响因子：
5
通讯作者：
Fan YJ
中科院分区：
文献类型：
--
作者：
Lu YJ;Hsieh HY;Kuo WC;Wei PK;Sheen HJ;Tahara H;Chiu TW;Fan YJ
本研究将局域化表面等离子体共振(LSPR)传感阵列与并行微流控通道相结合，提出了一种多路检测系统。在聚碳酸酯(PC)衬底上，采用纳米压痕和金溅射的方法制备了LSPR传感阵列。通过3-氨丙基三乙氧基硅烷(APTES)表面处理和氧等离子体处理，将聚二甲基硅氧烷(PDMS)微流控通道与PCLSPR传感阵列结合在一起。利用光谱仪对宽带白光照射和偏振波长测量获得了LSPR传感器件的共振光谱。用不同比例的甘油和不同折射率的水溶液测量了LSPR传感装置的灵敏度。用人免疫球蛋白G(Ig G)、Ig A和Ig M进行多重检测。在每个感受区分别修饰抗-Ig G、抗-Ig A和抗-Ig M。制备了不同浓度的人免疫球蛋白、免疫球蛋白A和免疫球蛋白M，以验证平行传感装置可用于检测不同目标的概念。
In this study, a multiplex detection system was proposed by integrating a localized surface plasmon resonance (LSPR) sensing array and parallel microfluidic channels. The LSPR sensing array was fabricated by nanoimprinting and gold sputter on a polycarbonate (PC) substrate. The polydimethylsiloxane (PDMS) microfluidic channels and PC LSPR sensing array were bound together through (3-aminopropyl)triethoxysilane (APTES) surface treatment and oxygen plasma treatment. The resonant spectrum of the LSPR sensing device was obtained by broadband white-light illumination and polarized wavelength measurements with a spectrometer. The sensitivity of the LSPR sensing device was measured using various ratios of glycerol to water solutions with different refractive indices. Multiplex detection was demonstrated using human immunoglobulin G (IgG), IgA, and IgM. The anti-IgG, anti-IgA, and anti-IgM were separately modified in each sensing region. Various concentrations of human IgG, IgA, and IgM were prepared to prove the concept that the parallel sensing device can be used to detect different targets.
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