“Mucosal maps” of the canine nasal cavity: Micro‐computed tomography and histology
“Mucosal maps” of the canine nasal cavity: Micro‐computed tomography and histology
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犬鼻腔的“粘膜图”：显微计算机断层扫描和组织学
DOI：
10.1002/ar.24511
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
DeLeon, Valerie B.
中科院分区：
文献类型：
--
作者：
Smith, Timothy D.;Craven, Brent A.;Engel, Serena M.;Van Valkenburgh, Blaire;DeLeon, Valerie B.
鼻鼻甲是鼻腔中支持呼吸或嗅觉粘膜（OM）的精致而复杂的骨骼，现在使用高分辨率微计算机断层扫描（μ‐CT）很容易研究。标准μ‐CT目前缺乏识别OM或其他粘膜类型的能力，如果没有额外的放射性不透明染色技术。然而，即使未染色的粘膜也比空气更不透明，因此可以辨别粘膜厚度。在这里，我们用30 μm的各向同性分辨率的μ‐CT扫描了一只成年狗的头骨，并对其进行了组织学切片和染色，以评估鼻窝的粘膜厚度。在μ - CT切片平面与组织学平面共对准后，估计了四个位置的粘膜厚度。基于μ - CT或组织学的结果表明，嗅觉粘膜比非嗅觉粘膜（non - OM）平均更厚。此外，嗅觉粘膜的变异性程度小于非嗅觉粘膜。后者的变异性似乎主要与固有层血管的不同程度有关。正因为如此，在具有特化血管呼吸粘膜和OM的结构中，如第一筛鼻（ET I）， OM和非OM的厚度范围可能重叠。未来的工作应该评估弥散性碘增强CT技术的效用，该技术可以区分上皮和固有层，以增强我们在更多的吻侧鼻翼上区分粘膜类型的能力。这对于像ET I这样的结构尤其重要，它在许多哺乳动物中具有混合的功能角色。
Nasal turbinals, delicate and complex bones of the nasal cavity that support respiratory or olfactory mucosa (OM), are now easily studied using high resolution micro‐computed tomography (μ‐CT). Standard μ‐CT currently lacks the capacity to identify OM or other mucosa types without additional radio‐opaque staining techniques. However, even unstained mucosa is more radio‐opaque than air, and thus mucosal thickness can be discerned. Here, we assess mucosal thickness of the nasal fossa using the cranium of a cadaveric adult dog that was μ‐CT scanned with an isotropic resolution of 30 μm, and subsequently histologically sectioned and stained. After co‐alignment of μ‐CT slice planes to that of histology, mucosal thickness was estimated at four locations. Results based on either μ‐CT or histology indicate olfactory mucosa is thicker on average compared with non‐olfactory mucosa (non‐OM). In addition, olfactory mucosa has a lesser degree of variability than the non‐OM. Variability in the latter appears to relate mostly to the varying degree of vascularity of the lamina propria. Because of this, in structures with both specialized vascular respiratory mucosa and OM, such as the first ethmoturbinal (ET I), the range of thickness of OM and non‐OM may overlap. Future work should assess the utility of diffusible iodine‐based contrast enhanced CT techniques, which can differentiate epithelium from the lamina propria, to enhance our ability to differentiate mucosa types on more rostral ethmoturbinals. This is especially critical for structures such as ET I, which have mixed functional roles in many mammals.
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DOI：
--
发表时间：
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期刊：
Journal of Physiology
影响因子：
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