A multielectrode array microchannel platform reveals both transient and slow changes in axonal conduction velocity.
A multielectrode array microchannel platform reveals both transient and slow changes in axonal conduction velocity.
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DOI：
10.1038/s41598-017-09033-3
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发表时间：
2017-08-17
影响因子：
4.6
通讯作者：
Blau A
中科院分区：
文献类型：
--
作者：
Habibey R;Latifi S;Mousavi H;Pesce M;Arab-Tehrany E;Blau A
由于尺寸小，对薄而复杂的轴突分支进行电生理学研究以支持理解它们在正常和患病大脑功能中的作用，这提出了实验挑战。为了降低实验复杂性，我们将微电极阵列（MEA）与双层微通道装置耦合，以高时空分辨率长期体外跟踪轴突形态和活动。我们的模型允许在显微镜兼容的环境中从纯轴突分支进行长期多位点记录。将网络结构划分为互连的子群，简化了对感兴趣位置的访问。 95 天的体外 (DIV) 电生理学数据显示，轴突传导速度呈年龄依赖性增加，与随时间变化的爆发活动呈正相关，但独立于爆发活动。在化学增加的网络活动水平下传导速度保持恒定。相比之下，轴突或网络亚群的低频（1赫兹，180次重复）电刺激会引起幅度依赖性直接（5-35毫秒周围刺激）和多突触（35-1,000毫秒周围刺激）活动，并沿着周围分支暂时（<35毫秒）提高传播速度。此外，与报道的无限制培养物（> 800 mV）相比，微通道中的有效刺激振幅明显较低（> 250 mV）。实验范式可能会对刺激引起的轴突可塑性产生新的见解。
Due to their small dimensions, electrophysiology on thin and intricate axonal branches in support of understanding their role in normal and diseased brain function poses experimental challenges. To reduce experimental complexity, we coupled microelectrode arrays (MEAs) to bi-level microchannel devices for the long-term in vitro tracking of axonal morphology and activity with high spatiotemporal resolution. Our model allowed the long-term multisite recording from pure axonal branches in a microscopy-compatible environment. Compartmentalizing the network structure into interconnected subpopulations simplified access to the locations of interest. Electrophysiological data over 95 days in vitro (DIV) showed an age-dependent increase of axonal conduction velocity, which was positively correlated with, but independent of evolving burst activity over time. Conduction velocity remained constant at chemically increased network activity levels. In contrast, low frequency (1 Hz, 180 repetitions) electrical stimulation of axons or network subpopulations evoked amplitude-dependent direct (5–35 ms peri-stimulus) and polysynaptic (35–1,000 ms peri-stimulus) activity with temporarily (<35 ms) elevated propagation velocities along the perisomatic branches. Furthermore, effective stimulation amplitudes were found to be significantly lower (>250 mV) in microchannels when compared with those reported for unconfined cultures (>800 mV). The experimental paradigm may lead to new insights into stimulation-induced axonal plasticity.
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影响因子：
4
作者：
Pan L;Alagapan S;Franca E;Brewer GJ;Wheeler BC
通讯作者：
Wheeler BC
影响因子：
4.3
作者：
Obien ME;Deligkaris K;Bullmann T;Bakkum DJ;Frey U
通讯作者：
Frey U
影响因子：
3.7
作者：
Biffi E;Regalia G;Menegon A;Ferrigno G;Pedrocchi A
通讯作者：
Pedrocchi A
平铺 3D 显微图像采集的全局最佳拼接。
DOI：
10.1093/bioinformatics/btp184
发表时间：
2009-06-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Preibisch S;Saalfeld S;Tomancak P
通讯作者：
Tomancak P
影响因子：
4.3
作者：
Lewandowska MK;Radivojević M;Jäckel D;Müller J;Hierlemann AR
通讯作者：
Hierlemann AR