Propagation of action potential activity in a predefined microtunnel neural network.
Propagation of action potential activity in a predefined microtunnel neural network.
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DOI：
10.1088/1741-2560/8/4/046031
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发表时间：
2011-08
影响因子：
4
通讯作者：
Wheeler BC
中科院分区：
文献类型：
--
作者：
Pan L;Alagapan S;Franca E;Brewer GJ;Wheeler BC
将具有两个威尔斯孔的聚二甲基硅氧烷（PDMS）微隧道装置对准并附着在多电极阵列（MEA）的顶部。神经元首先在一个孔中生长，并允许轴突通过隧道传播到第二个孔中。十天后，将细胞接种在第二孔中，轴突延伸回到第一孔的可能性低得多，目的是在两个威尔斯孔中的神经元群体之间产生单向连接。在这里，我们报告的电生理证据支持的假设，占主导地位的信息流是在所需的方向。这是通过测量单个动作电位的传播速度和方向来完成的，结果是84%的尖峰在期望的方向上传播。此外，我们在每个电极上记录了全局同步的爆发活动，确定了每个电极上第一个尖峰的时间，记录了仅在第二个孔中发现的局部同步爆发活动，并且不会传播回第一个孔，并得出结论，这种爆发传播的测量支持单向连接网络的假设。两个假设讨论的机制，潜在的特定的神经网络的活动模式。
A polydimethylsiloxane (PDMS) microtunnel device with two wells is aligned and attached on top of a multi-electrode-array (MEA). Neurons are grown first in one well and allowed to propagate axons through the tunnels into a second well. After ten days cells are plated in the second well, with much lower likelihood of extending axons back to the first well, with the intent of creating unidirectional connectivity between populations of neurons in the two wells. Here we report electrophysiological evidence that supports the hypothesis that the dominant information flow is in the desired direction. This was done by measuring the propagation speed and direction of individual action potentials, with the result that 84% of the spikes propagated in the desired direction. Further, we recorded globally synchronized burst activity on each of the electrodes, identified the timing of the first spike on each electrode, recorded locally synchronized burst activity which is found only in the second well and does not propagate back to the first well, and concluded that this measure of burst propagation supports the hypothesis of a unidirectionally connected network. Two hypotheses are discussed for mechanism underlying the activity pattern of the particular neural networks.
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DOI：
10.1523/jneurosci.6130-08.2009
发表时间：
2009-04-15
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
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通讯作者：
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影响因子：
4
作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
10.1049/ip-nbt:20040706
发表时间：
2004-06-01
期刊：
IEE proceedings. Nanobiotechnology
影响因子：
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作者：
Nam, Y;Chang, J;Wheeler, B C
通讯作者：
Wheeler, B C
影响因子：
6.1
作者：
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通讯作者：
Frazier, A. Bruno