Quantitative analysis of nanoparticle internalization in mammalian cells by high resolution X-ray microscopy.
Quantitative analysis of nanoparticle internalization in mammalian cells by high resolution X-ray microscopy.
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DOI：
10.1186/1477-3155-9-14
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发表时间：
2011-04-10
影响因子：
10.2
通讯作者：
Margaritondo G
中科院分区：
文献类型：
--
作者：
Chen HH;Chien CC;Petibois C;Wang CL;Chu YS;Lai SF;Hua TE;Chen YY;Cai X;Kempson IM;Hwu Y;Margaritondo G
细胞水平上纳米颗粒摄取的定量分析对于纳米医学程序至关重要。特别是需要对其效果进行现实评估。不幸的是，纳米颗粒吸收的定量测量仍然面临着巨大的技术挑战。我们在这里提出了一种解决这个问题的方法，并分析不同类型细胞中存在的金属纳米粒子的数量。该方法依赖于具有吸收对比度和相位对比度的高横向分辨率（优于 30 nm）透射 X 射线微图像，包括直接显示纳米颗粒的二维 (2D) 投影图像和三维 (3D) 断层扫描重建。对通过 X 射线照射获得的裸金纳米粒子和聚乙二醇 (PEG) 涂覆的金纳米粒子成功进行了实际测试。使用两种不同的细胞系 EMT 和 HeLa，我们获得了每个细胞摄取的纳米颗粒簇的数量和簇的大小。此外，分析还揭示了 2D 和 3D 培养细胞之间以及同一 3D 样本的 2D 和 3D 数据之间的有趣差异。我们证明了我们的方法的可行性和有效性，证明它足以准确地测量细胞之间的纳米颗粒摄取差异以及形成的纳米颗粒簇的尺寸。 2D 和 3D 文化以及 2D 和 3D 图像之间的差异强调了 3D 分析的重要性，而我们的方法使 3D 分析成为可能。
Quantitative analysis of nanoparticle uptake at the cellular level is critical to nanomedicine procedures. In particular, it is required for a realistic evaluation of their effects. Unfortunately, quantitative measurements of nanoparticle uptake still pose a formidable technical challenge. We present here a method to tackle this problem and analyze the number of metal nanoparticles present in different types of cells. The method relies on high-lateral-resolution (better than 30 nm) transmission x-ray microimages with both absorption contrast and phase contrast -- including two-dimensional (2D) projection images and three-dimensional (3D) tomographic reconstructions that directly show the nanoparticles. Practical tests were successfully conducted on bare and polyethylene glycol (PEG) coated gold nanoparticles obtained by x-ray irradiation. Using two different cell lines, EMT and HeLa, we obtained the number of nanoparticle clusters uptaken by each cell and the cluster size. Furthermore, the analysis revealed interesting differences between 2D and 3D cultured cells as well as between 2D and 3D data for the same 3D specimen. We demonstrated the feasibility and effectiveness of our method, proving that it is accurate enough to measure the nanoparticle uptake differences between cells as well as the sizes of the formed nanoparticle clusters. The differences between 2D and 3D cultures and 2D and 3D images stress the importance of the 3D analysis which is made possible by our approach.
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影响因子：
13.6
作者：
Chen ZG
通讯作者：
Chen ZG
影响因子：
11.2
作者：
Passarella RJ;Spratt DE;van der Ende AE;Phillips JG;Wu H;Sathiyakumar V;Zhou L;Hallahan DE;Harth E;Diaz R
通讯作者：
Diaz R
影响因子：
3.3
作者：
Mahmood, Meena;Casciano, Daniel A.;Biris, Alexandru S.
通讯作者：
Biris, Alexandru S.
影响因子：
3.5
作者：
Cai, Xiaoqing;Wang, Cheng-Liang;Margaritondo, G.
通讯作者：
Margaritondo, G.
影响因子：
16.1
作者：
Janib, Siti M.;Moses, Ara S.;MacKay, J. Andrew
通讯作者：
MacKay, J. Andrew