Comparative Methylome Analysis Reveals Perturbation of Host Epigenome in Chestnut Blight Fungus by a Hypovirus.
Comparative Methylome Analysis Reveals Perturbation of Host Epigenome in Chestnut Blight Fungus by a Hypovirus.
复制标题
比较甲基化分析揭示了低病毒对板栗枯萎病真菌宿主表观基因组的干扰。
DOI：
10.3389/fmicb.2018.01026
复制
发表时间：
2018
影响因子：
5.2
通讯作者：
Chen B
中科院分区：
文献类型：
--
作者：
Li R;Zhou S;Li Y;Shen X;Wang Z;Chen B
在真核生物基因组中，DNA甲基化是一种重要的表观遗传修饰，在许多生物过程中起着至关重要的作用。为了研究低病毒感染对板栗疫病菌寄生Cryphonectria parasitica甲基组的影响，采用全基因组亚硫酸盐测序（WGBS）技术对感染/未感染板栗疫病菌的单碱基分辨率甲基组进行了分析。结果表明，次病毒感染改变了所有三种情况（CG， CHG和CHH）的甲基化，尤其是基因启动子。共鉴定出600个差异甲基化区（DMRs），其中144个可以被注释为功能基因。RNA-seq分析显示，启动子DNA甲基化与基因表达呈负相关。在DMRs中，有4个基因与分生、橙色色素产生和毒力有关。总之，我们的DNA甲基组分析为理解DNA甲基化与低病毒感染之间的关系以及寄生蜂的表型性状提供了有价值的见解。
In eukaryotic genomes, DNA methylation is an important type of epigenetic modification that plays crucial roles in many biological processes. To investigate the impact of a hypovirus infection on the methylome of Cryphonectria parasitica, the chestnut blight fungus, whole-genome bisulfite sequencing (WGBS) was employed to generate single-base resolution methylomes of the fungus with/without hypovirus infection. The results showed that hypovirus infection alters methylation in all three contexts (CG, CHG, and CHH), especially in gene promoters. A total of 600 differentially methylated regions (DMRs) were identified, of which 144 could be annotated to functional genes. RNA-seq analysis revealed that DNA methylation in promoter is negatively correlated with gene expression. Among DMRs, four genes were shown to be involved in conidiation, orange pigment production, and virulence. Taken together, our DNA methylomes analysis provide valuable insights into the understanding of the relationship between DNA methylation and hypovirus infection, as well as phenotypic traits in C. parasitica.
登录
查看更多内容
影响因子：
4.9
作者：
Chen MM;Jiang M;Shang J;Lan X;Yang F;Huang J;Nuss DL;Chen B
通讯作者：
Chen B
影响因子：
4.3
作者：
Conerly, Melissa;Grady, William M.
通讯作者：
Grady, William M.
影响因子：
4.6
作者：
Hao Y;Cui Y;Gu X
通讯作者：
Gu X
影响因子：
2.8
作者：
Dawe, Angus L.;Van Voorhies, Wayne A.;Li, Zhong
通讯作者：
Li, Zhong
影响因子：
--
作者：
Allen, TD;Dawe, AL;Nuss, DL
通讯作者：
Nuss, DL