Isolation of Retinal Arterioles for Ex Vivo Cell Physiology Studies.
Isolation of Retinal Arterioles for Ex Vivo Cell Physiology Studies.
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分离视网膜小动脉用于离体细胞生理学研究。
DOI：
10.3791/57944
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发表时间：
2018-07-14
期刊：
影响因子：
--
通讯作者：
McGahon MK
中科院分区：
文献类型：
--
作者：
Curtis TM;McLaughlin D;O'Hare M;Kur J;Barabas P;Revolta G;Scholfield CN;McGeown JG;McGahon MK
视网膜是一个代谢高度活跃的组织，需要大量的血液供应。视网膜循环支持视网膜内部，而脉络膜血管则供应感光器。视网膜灌注的改变会导致许多威胁视力的疾病，包括糖尿病性视网膜病变、青光眼和视网膜分支静脉阻塞。了解控制通过视网膜的血流的分子机制以及这些机制在眼部疾病期间如何改变可能有助于确定治疗这些疾病的新靶标。视网膜小动脉是视网膜的主要阻力血管，因此通过改变管腔直径在调节视网膜血流动力学中发挥关键作用。近年来，我们开发了从大鼠视网膜中分离小动脉的方法，该方法适用于包括细胞生理学研究在内的广泛应用。这种准备工作已经开始对视网膜血流如何控制产生新的见解，并使我们能够识别眼部疾病期间发生的一些关键变化。在本文中，我们描述了大鼠视网膜小动脉的分离方法，并包括其在膜片钳电生理学、钙成像和压力肌动描记研究中使用的方案。这些容器还适合用于基于 PCR、蛋白质印迹和免疫组织化学的研究。
The retina is a highly metabolically active tissue that requires a substantial blood supply. The retinal circulation supports the inner retina, while the choroidal vessels supply the photoreceptors. Alterations in retinal perfusion contribute to numerous sight-threatening disorders, including diabetic retinopathy, glaucoma and retinal branch vein occlusions. Understanding the molecular mechanisms involved in the control of blood flow through the retina and how these are altered during ocular disease could lead to the identification of new targets for the treatment of these conditions. Retinal arterioles are the main resistance vessels of the retina, and consequently, play a key role in regulating retinal hemodynamics through changes in luminal diameter. In recent years, we have developed methods for isolating arterioles from the rat retina which are suitable for a wide range of applications including cell physiology studies. This preparation has already begun to yield new insights into how blood flow is controlled in the retina and has allowed us to identify some of the key changes that occur during ocular disease. In this article, we describe methods for the isolation of rat retinal arterioles and include protocols for their use in patch-clamp electrophysiology, calcium imaging and pressure myography studies. These vessels are also amenable for use in PCR-, western blotting- and immunohistochemistry-based studies.
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影响因子：
17.8
作者：
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通讯作者：
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影响因子：
5.5
作者：
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影响因子：
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通讯作者：
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作者：
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通讯作者：
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