A novel genome of C and the first autotetraploid species in the Setaria genus identified by genomic in situ hybridization

A novel genome of C and the first autotetraploid species in the Setaria genus identified by genomic in situ hybridization
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通过基因组原位杂交鉴定出 C 的新基因组和狗尾草属中第一个同源四倍体物种

DOI:
10.1007/s10722-009-9405-7
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发表时间:
2009-02
影响因子:
2
通讯作者:
Huang, Zhanjing
Huang, Zhanjing
中科院分区:
农林科学3区
文献类型:
--
作者:
Zhi, Hui;Li, Wei;Li, Haiquan;Wang, Yongqiang;Wang, Yongfang;Diao, Xianmin;Huang, Zhanjing

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利用基因组原位杂交技术研究了狗尾草属部分新种的基因组亲缘关系。以前的工作表明,S。viridis和S.粘粒分别携带基因组A和B。本研究中获得的GISH图谱清楚地区分了S. grisebachii与已知基因组A和B的同源性进行了比较,并指出了其新的基因组组成,建议将其命名为狗尾草属基因组C。四倍体S. queenslandica与S.表明其同源四倍体的性质。这是首次在狗尾草属植物中发现同源四倍体,应将狗尾草属植物归入初级A基因组基因库,而不是以前划分的三级基因库。GISH图谱不能区分S. leucopila的A基因组。viridis和S. italica,表明其密切关系与foxtail小米。当S.以adhaerens基因组DNA为探针,对二倍体S. verticillata,推测其基因组性质为B。结合形态学观察和前人的工作,我们推测二倍体S. verticillata和S. adhaerens在分类学上可能是名称不同的同一物种。
Genomic in situ hybridization (GISH) was used to investigate the genomic relationships among some newly collected species of genus Setaria. Previous work identified that S. viridis and S. adhaerens carry genomes A and B, respectively. GISH patterns obtained in this report clearly distinguished the genome of S. grisebachii from the known genomes A and B, and indicated its new genomic constitution which we suggest to name genome C of the Setaria genus. The two sets of chromosomes of tetraploid S. queenslandica hybridized well with the A genome of S. viridis indicating its autotetraploid nature. This is the first autotetraploid identified in the Setaria genus, which should be classified into the primary A genome gene pool rather than the tertiary gene pool as previously classified. GISH patterns did not distinguish the genome of S. leucopila from the A genome of S. viridis and S. italica, suggesting its close relation with foxtail millet. Strong hybridization signals were observed when S. adhaerens genomic DNA was used as probe to hybridize the chromosomes of diploid S. verticillata, inferring its B genome nature. Combined with morphological observation and previous work, we deduce that diploid S. verticillata and S. adhaerens are probably taxonomically the same species with different names.
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