Virulence differences between monkeypox virus isolates from West Africa and the Congo basin.
Virulence differences between monkeypox virus isolates from West Africa and the Congo basin.
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DOI：
10.1016/j.virol.2005.05.030
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发表时间：
2005-09-15
期刊：
影响因子：
3.7
通讯作者：
Buller, RML
中科院分区：
文献类型：
--
作者：
Chen, NH;Li, GY;Liszewski, MK;Atkinson, JP;Jahrling, PB;Feng, ZH;Schriewer, J;Buck, C;Wang, CL;Lefkowitz, EJ;Esposito, JJ;Harms, T;Damon, IK;Roper, RL;Upton, C;Buller, RML
研究表明，西非和刚果盆地分离的猴痘病毒（MPXV）在遗传上是不同的。在这里，我们显示刚果盆地MPXV-ZAI-V79与假定的西非MPXV-COP-58相比对食蟹猴更具毒性。这一发现可以解释为什么美国2003年猴痘疫情中没有死亡病例，猴痘疫情是由西非病毒引起的。西非和刚果盆地MPXV之间的毒力差异得到流行病学分析的进一步支持，流行病学分析观察到两个地区未接种疫苗人群中的抗体流行率相似，而>90%的报告病例发生在刚果盆地，在该地区以外未观察到致死性病例。为了确定这种毒力差异的基础，我们对一种西非人分离株和两种假定的西非分离株的基因组进行了测序，并将序列与刚果盆地MPXV-ZAI-96-I-16进行了比较。该分析鉴定了D10 L、D14 L、B10 R、B14 R和B19 R为可能的毒力基因，其中D14 L（牛痘补体蛋白的直系同源物）为主要候选基因。
Studies indicate that West African and Congo basin isolates of monkeypox virus (MPXV) are genetically distinct. Here, we show Congo basin MPXV-ZAI-V79 is more virulent for cynomolgus monkeys as compared to presumed West African MPXV-COP-58. This finding may explain the lack of case-fatalities in the U.S. 2003 monkeypox outbreak, which was caused by a West African virus. Virulence differences between West African and Congo basin MPXV are further supported by epidemiological analyses that observed a similar prevalence of antibodies in non-vaccinated humans in both regions, while >90% of reported cases occurred in the Congo basin, and no fatal cases were observed outside of this region. To determine the basis for this difference in virulence, we sequenced the genomes of one human West African isolate, and two presumed West African isolates and compared the sequences to Congo basin MPXV-ZAI-96-I-16. The analysis identified D10L, D14L, B10R, B14R, and B19R as possible virulence genes, with D14L (ortholog of vaccinia complement protein) as a leading candidate.
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