The Tail-Specific Protease Is Important for Legionella pneumophila To Survive Thermal Stress in Water and inside Amoebae
The Tail-Specific Protease Is Important for Legionella pneumophila To Survive Thermal Stress in Water and inside Amoebae
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尾部特异性蛋白酶对于嗜肺军团菌在水中和阿米巴体内的热应激生存非常重要
DOI：
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发表时间：
2021
影响因子：
4.4
通讯作者：
S. Faucher
中科院分区：
文献类型：
--
作者：
Joseph Saoud;Thangadurai Mani;S. Faucher
脂蛋白是医院获得性和社区获得性肺炎的主要原因。Lp存在于水系统中，包括热水分配系统。热处理是一种消毒方法，通常用于限制此类系统中Lp的存在;然而，这种益处通常是短期的，因为Lp能够快速使这些系统酸化。摘要嗜肺军团菌（Legionella pneumophila，Lp）是天然和人造水体中的一种寄生菌，它在阿米巴原虫和纤毛虫中复制，并在生物膜中存活。当吸入受Lp污染的气溶胶时，Lp可以进入肺部并可能感染人的肺泡巨噬细胞，引起称为军团病的严重肺炎。Lp经常在热水分配系统（HWDS）中发现，这与医院爆发有关。热处理用于消毒HWDS并降低Lp浓度。然而，Lp通常能够使这些水系统酸化，表明有效的热休克反应。尾特异性蛋白酶（Tsp）通常是涉及降解周质中的异常蛋白的周质蛋白酶，并且对于在热应激下存活是重要的。在Lp Philadelphia-1中，Tsp由lpg 0499基因编码。在本文中，我们表明，TSP是重要的生存在水中的热应力和最佳感染的变形虫时，在细胞内生长过程中发生的温度变化。我们还表明，TSP的表达在postexponential阶段，但在指数期抑制，顺式编码的小调控RNA Lpr 17显示相反的表达，这表明它抑制TSP的翻译。此外，我们的研究结果表明，tsp是由CpxR，一个主要的调节Lp，在Lpr 17独立的方式。CpxR的缺失也降低了Lp在热休克中存活的能力。总之，我们的研究表明，Tsp可能是水体中Lp生存和生长的重要因素。重要性Lp是医院获得性和社区获得性肺炎的主要原因。Lp存在于水系统中，包括热水分配系统。热处理是一种消毒方法，通常用于限制此类系统中Lp的存在;然而，这种益处通常是短期的，因为Lp能够快速使这些系统酸化。据推测，脂蛋白对热应激反应有效，但到目前为止，对相关基因的了解还不多。在本文中，我们表明，Tsp和双组分系统CpxRA是所需的热应力时，Lp是免费的水，当它是在宿主细胞内的电阻。我们的研究确定了关键系统的LP在其自然环境下的热应力下的生存。
Lp is a major cause of nosocomial and community-acquired pneumonia. Lp is found in water systems, including hot water distribution systems. Heat treatment is a method of disinfection often used to limit the presence of Lp in such systems; however, the benefit is usually short term, as Lp is able to quickly recolonize these systems. ABSTRACT Legionella pneumophila (Lp) is an inhabitant of natural and human-made water systems, where it replicates within amoebae and ciliates and survives within biofilms. When Lp-contaminated aerosols are breathed in, Lp can enter the lungs and may infect human alveolar macrophages, causing severe pneumonia known as Legionnaires’ disease. Lp is often found in hot water distribution systems (HWDS), which are linked to nosocomial outbreaks. Heat treatment is used to disinfect HWDS and reduce the concentration of Lp. However, Lp is often able to recolonize these water systems, indicating an efficient heat shock response. Tail-specific proteases (Tsp) are typically periplasmic proteases implicated in degrading aberrant proteins in the periplasm and important for surviving thermal stress. In Lp Philadelphia-1, Tsp is encoded by the lpg0499 gene. In this paper, we show that Tsp is important for surviving thermal stress in water and for optimal infection of amoeba when a shift in temperature occurs during intracellular growth. We also demonstrate that Tsp is expressed in the postexponential phase but repressed in the exponential phase and that the cis-encoded small regulatory RNA Lpr17 shows the opposite expression, suggesting that it represses translation of tsp. In addition, our results show that tsp is regulated by CpxR, a major regulator in Lp, in an Lpr17-independent manner. Deletion of CpxR also reduced the ability of Lp to survive heat shock. In conclusion, our study shows that Tsp is likely an important factor for the survival and growth of Lp in water systems. IMPORTANCE Lp is a major cause of nosocomial and community-acquired pneumonia. Lp is found in water systems, including hot water distribution systems. Heat treatment is a method of disinfection often used to limit the presence of Lp in such systems; however, the benefit is usually short term, as Lp is able to quickly recolonize these systems. Presumably, Lp responds efficiently to thermal stress, but so far, not much is known about the genes involved. In this paper, we show that the Tsp and the two-component system CpxRA are required for resistance to thermal stress when Lp is free in water and when it is inside host cells. Our study identifies critical systems for the survival of Lp in its natural environment under thermal stress.
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