Cross-Talk Between Butyric Acid and Gut Microbiota in Ulcerative Colitis Following Fecal Microbiota Transplantation.
Cross-Talk Between Butyric Acid and Gut Microbiota in Ulcerative Colitis Following Fecal Microbiota Transplantation.
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粪便微生物群移植后溃疡性结肠炎中丁酸与肠道微生物群的相互作用
DOI：
10.3389/fmicb.2021.658292
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发表时间：
2021
影响因子：
5.2
通讯作者：
Nie YQ
中科院分区：
文献类型：
--
作者：
Xu HM;Huang HL;Xu J;He J;Zhao C;Peng Y;Zhao HL;Huang WQ;Cao CY;Zhou YJ;Zhou YL;Nie YQ
粪便微生物区系移植(FMT)可以抑制溃疡性结肠炎(UC)的进展。然而，FMT是如何调节肠道微生物区系的，以及哪些生物标志物对评估FMT的疗效有价值，目前还不清楚。本研究旨在确定UC患者FMT后肠道微生物区系的变化及其与丁酸的关系。从健康人或小鼠中分离出粪便微生物区系(FM)，并将其移植到12例UC患者或葡聚糖硫酸钠(DSS)诱导的结肠炎小鼠体内。监测他们的临床结肠炎严重程度。用16S测序和生物信息学方法对其肠道微生物区系进行分析。用液相色谱-质谱法(LC-MS)对5例FMT后复发的UC患者和单个小鼠的粪便短链脂肪酸(SCFAs)水平进行了测定。体外试验了丁酸对肠道微生物区系丰度和多样性的影响。回顾分析丁酸菌联合FMT对45例UC患者临床疗效的影响。与对照组相比，FMT显著增加了UC患者的丁酸产生菌数量和粪丁酸水平。FMT显著增加了结肠炎小鼠的α多样性，改变了肠道微生物结构，并提高了粪便丁酸水平。添加丁酸的厌氧培养显著增加了结肠炎小鼠肠道微生物区系的α多样性，并改变了它们的结构。FMT联合含酪酸梭菌的益生菌可显著延长UC的临床缓解时间。因此，粪便丁酸水平可能是评价FMT治疗UC疗效的一个生物标志物，而添加丁酸产生菌可能通过改变肠道菌群来延长FMT对UC的疗效。
Fecal microbiota transplantation (FMT) can inhibit the progression of ulcerative colitis (UC). However, how FMT modulates the gut microbiota and which biomarker is valuable for evaluating the efficacy of FMT have not been clarified. This study aimed to determine the changes in the gut microbiota and their relationship with butyric acid following FMT for UC. Fecal microbiota (FM) was isolated from healthy individuals or mice and transplanted into 12 UC patients or colitis mice induced by dextran sulfate sodium (DSS). Their clinical colitis severities were monitored. Their gut microbiota were analyzed by 16S sequencing and bioinformatics. The levels of fecal short-chain fatty acids (SCFAs) from five UC patients with recurrent symptoms after FMT and individual mice were quantified by liquid chromatography–mass spectrometry (LC–MS). The impact of butyric acid on the abundance and diversity of the gut microbiota was tested in vitro. The effect of the combination of butyric acid-producing bacterium and FMT on the clinical responses of 45 UC patients was retrospectively analyzed. Compared with that in the controls, the FMT significantly increased the abundance of butyric acid-producing bacteria and fecal butyric acid levels in UC patients. The FMT significantly increased the α-diversity, changed gut microbial structure, and elevated fecal butyric acid levels in colitis mice. Anaerobic culture with butyrate significantly increased the α-diversity of the gut microbiota from colitis mice and changed their structure. FMT combination with Clostridium butyricum-containing probiotics significantly prolonged the UC remission in the clinic. Therefore, fecal butyric acid level may be a biomarker for evaluating the efficacy of FMT for UC, and addition of butyrate-producing bacteria may prolong the therapeutic effect of FMT on UC by changing the gut microbiota.
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影响因子：
7.4
作者：
Cui B;Li P;Xu L;Zhao Y;Wang H;Peng Z;Xu H;Xiang J;He Z;Zhang T;Nie Y;Wu K;Fan D;Ji G;Zhang F
通讯作者：
Zhang F
影响因子：
14.9
作者：
Pruesse E;Quast C;Knittel K;Fuchs BM;Ludwig W;Peplies J;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
28.3
作者：
Lagier, Jean-Christophe;Khelaifia, Saber;Raoult, Didier
通讯作者：
Raoult, Didier
影响因子：
14.8
作者：
Wirtz, Stefan;Neufert, Clemens;Neurath, Markus F.
通讯作者：
Neurath, Markus F.
影响因子：
7.3
作者：
Guo M;Wu F;Hao G;Qi Q;Li R;Li N;Wei L;Chai T
通讯作者：
Chai T