Rescue effects of IPE transplants in RCS rats: short-term results.
Rescue effects of IPE transplants in RCS rats: short-term results.
复制标题
IPE 移植对 RCS 大鼠的拯救作用：短期结果。
DOI：
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复制
发表时间：
1999
影响因子：
4.4
通讯作者：
Klaus Heimann
中科院分区：
文献类型：
--
作者：
Ulrich Schraermeyer;Norbert Kociok;Klaus Heimann
目的
本研究旨在通过光镜和电子显微镜组织学方法观察视网膜下虹膜色素上皮(IPE)细胞移植对皇家外科学院(RCS)大鼠的挽救作用。
方法
IPE细胞取自出生20至26天的Long-Evans大鼠，使用32号哈密尔顿注射器直接移植到32只出生16至20天的RCS大鼠的视网膜下间隙。移植眼8周后取材包埋，行电子显微镜检查。取Long-Evans大鼠的虹膜和视网膜色素上皮(RPE)标本以及未经手术治疗的RCS大鼠的RPE标本。假手术8只眼。
结果
移植到视网膜下间隙的IPE细胞定位于宿主RPE和视网膜之间，细胞呈圆形，无极组织，含有杆状外节摄取的吞噬小体。宿主RPE细胞色素沉着。RCS大鼠RPE细胞内质网碎裂，在超微结构上有别于Long-Evans大鼠的色素上皮细胞。移植细胞胞浆内可见超微结构改变。IPE细胞中的黑色素颗粒存在于大的空泡中，空泡中还含有摄取ROS的吞噬小体。术后2个月，13只眼中6只眼(46%)和4只眼(31%)移植的IPE细胞表面分别检测到1~4排和5~8排保留的感光细胞。10只眼(53%)和7只眼(37%)在切面无IPE细胞的位置分别有1~4行和5~8行。1例可见针对移植的IPE细胞的ROS，但这些视杆变短且排列紊乱。在移植细胞和感光细胞内段之间的大多数部位，没有外段和细胞碎片。没有移植细胞的眼在2个月大时没有光感受器细胞存活。假手术后，6只眼(75%)有1~4排感光细胞，2只眼(25%)有5~8排感光细胞。
结论
移植的IPE细胞可在RCS大鼠体内摄取和降解ROS。ROS的摄取改变了移植的IPE细胞中色素颗粒的形态。色素沉着是识别移植的色素细胞的一个不确定的标志。在挽救光感受器方面，IPE移植不如RPE移植。然而，移植眼和未治疗眼之间存在显著差异。IPE细胞的救治效果与假手术无明显差异。
PURPOSE
The aim of this study was to investigate the possible rescue effect of subretinal iris pigment epithelial (IPE) cell transplantation in Royal College of Surgeons (RCS) rats by light and electron microscopic histology.
METHODS
IPE cells were harvested from 20- to 26-day-old Long-Evans rats and were directly trans planted transsclerally into the subretinal space of 32 16- to 20-day-old RCS rats using a 32-gauge Hamilton syringe. Specimens of transplanted eyes were embedded for electron microscopy after 8 weeks. Specimens from the iris and retinal pigment epithelium (RPE) of Long-Evans rats and RPE from RCS rats without surgical treatment were also embedded. Sham surgery was also performed in 8 eyes.
RESULTS
The IPE cells transplanted into the subretinal space were localized between host RPE and retina, had round cell shapes without polar organization, and contained phagosomes resulting from rod outer segment (ROS) uptake. The underlying host RPE cells were heavily pigmented. RPE cells from RCS rats revealed fragmentation of endoplasmic reticulum, which distinguishes them ultrastructurally from pigment epithelial cells of Long-Evans rats. Ultrastructural alterations were observed in the cytoplasm of transplanted cells. Melanin granules in the IPE cells were found in large vacuoles, which also contained phagosomes originating from ROS uptake. In 13 eyes, 1 to 4 rows and 5 to 8 rows of saved photoreceptors were detected facing transplanted IPE cells in 6 (46%) and 4 (31%) eyes, respectively, 2 months after surgery. However, in 10 (53%) and 7 (37%) of 19 eyes, 1 to 4 rows and 5 to 8 rows, respectively, were also found at sites without IPE cells in the plane of section. ROS directed toward transplanted IPE cells were seen in one case, but these rods were shortened and disorganized. At most sites between transplanted cells and inner segments of photoreceptors, outer segments and cellular debris were absent. In eyes without transplanted cells no photoreceptor cells were alive at the age of 2 months. After sham surgery 6 (75%) eyes had 1 to 4 rows and 2 (25%) 5 to 8 rows of photoreceptors.
CONCLUSIONS
Transplanted IPE cells can take up and degrade ROS in vivo in RCS rats. Uptake of ROS alters the morphology of pigment granules in transplanted IPE cells. Pigmentation is an uncertain marker for identifying transplanted pigment cells. IPE transplants are not as good as RPE transplants in rescuing photoreceptors. However, there is a significant difference between transplanted eyes and nontreated eyes. The rescue effect of IPE cells was not significantly different from that of sham surgery.
营养不良的大鼠视网膜中，血管密度和玻璃体视网膜膜的增加伴随着色素上皮的血管化。
影响因子：
2
作者：
Caldwell,RB;Roque,RS;Solomon,SW
通讯作者：
Solomon,SW
影响因子：
4.4
作者：
S. Yamamoto;J. Du;P. Gouras;H. Kjeldbye
通讯作者：
S. Yamamoto;J. Du;P. Gouras;H. Kjeldbye
溶酶体相关膜蛋白-1 (LAMP-1) 是黑素细胞囊泡膜糖蛋白带 II。
DOI：
10.1111/1523-1747.ep12462775
发表时间：
1993
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Zhou,BK;Boissy,RE;Pifko-Hirst,S;Moran,DJ;Orlow,SJ
通讯作者：
Orlow,SJ