Hydroxyapatite nanoparticle reinforced peptide amphiphile nanomatrix enhances the osteogenic differentiation of mesenchymal stem cells by compositional ratios.
Hydroxyapatite nanoparticle reinforced peptide amphiphile nanomatrix enhances the osteogenic differentiation of mesenchymal stem cells by compositional ratios.
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DOI：
10.1016/j.actbio.2012.07.024
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发表时间：
2012-11
影响因子：
9.7
通讯作者：
Jun, Ho-Wook
中科院分区：
文献类型：
--
作者：
Vines, Jeremy B.;Lim, Dong-Jin;Anderson, Joel M.;Jun, Ho-Wook
关键词：
在骨组织工程领域，需要模拟天然骨细胞外基质（ECM）的材料。这种需求通过创建旨在模拟天然骨ECM的有机和无机方面的双相复合材料来满足。然而，很少有研究创造了具有能够指导细胞行为的有机ECM类似成分的复合材料，并且许多都不是以纳米级制造的。此外，很少有人试图调查有机和无机成分的变化如何影响人间充质干细胞（hMSCs）的成骨分化。为了解决这些问题，以各种HANP与PA-RGDS的比率创建了由嵌入用RGDS细胞粘附基序定制的肽两亲物（PA）纳米纤维内的羟基磷灰石纳米颗粒（HANP）组成的双相纳米基质复合材料（PA-RGDS）。通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）证实了这些双相纳米基质复合材料的制备。然后通过定量指示成骨分化、碱性磷酸酶活性和DNA含量的基因随时间的定时表达来评估hMSC响应于不同组成比例的长期细胞性和成骨分化。随着时间的推移，细胞结构和基因表达的减少与HANP和PA-RGDS之间的组成比增加相关。最高的HANP与PA-RGDS比率（66%HANP）表现出对hMSCs的成骨分化的最大改善。总之，这些结果表明，双相纳米基质复合物的组成比例在影响hMSC的成骨分化中起重要作用。基于本研究中提出的观察结果，这些双相纳米基质复合材料显示出在骨组织工程应用中未来使用的前景。
In the field of bone tissue engineering, there is a need for materials that mimic the native bone extracellular matrix (ECM). This need is met through the creation of biphasic composites intended to mimic both the organic and inorganic facets of the native bone ECM. However, few studies have created composites with organic ECM analogous components capable of directing cellular behaviors and many are not fabricated in the nanoscale. Furthermore, few attempts have been made at investigating how variations of organic and inorganic components affect the osteogenic differentiation of human mesenchymal stem cells (hMSCs). To address these issues, biphasic nanomatrix composites consisting of hydroxyapatite nanoparticles (HANPs) embedded within peptide amphiphile (PA) nanofibers tailored with the RGDS cellular adhesion motif (PA-RGDS) were created at various HANP to PA-RGDS ratios. Fabrication of these biphasic nanomatrix composites were confirmed via scanning electron microscope (SEM) and transmission electron microscope (TEM). The long-term cellularity and osteogenic differentiation of hMSCs in response to the different compositional ratios was then assessed by quantifying the timed expression of genes indicative of osteogenic differentiation, alkaline phosphatase activity, and DNA content over time. Decreased cellularity and the expression of genes over time correlated with increasing compositional ratios between HANP and PA-RGDS. The highest HANP to PA-RGDS ratio (66% HANP) exhibited the greatest improvement to the osteogenic differentiation of hMSCs. Overall, these results demonstrate that the compositional ratio of biphasic nanomatrix composites plays an important role in influencing the osteogenic differentiation of hMSCs. Based on the observations presented within this study, these biphasic nanomatrix composites show promise for future usage in bone tissue engineering applications.
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影响因子：
6.2
作者：
Anderson, Joel M.;Kushwaha, Meenakshi;Tambralli, Ajay;Bellis, Susan L.;Camata, Renato P.;Jun, Ho-Wook
通讯作者：
Jun, Ho-Wook
影响因子：
14
作者：
Barker, Thomas H.
通讯作者：
Barker, Thomas H.
影响因子：
4.1
作者：
He, Jiawei;Genetos, Damian C.;Leach, J. Kent
通讯作者：
Leach, J. Kent
影响因子：
14
作者：
Hennessy, Kristin M.;Pollot, Beth E.;Clem, William C.;Phipps, Matthew C.;Sawyer, Amber A.;Culpepper, Bonnie K.;Bellis, Susan L.
通讯作者：
Bellis, Susan L.
DOI：
10.1023/a:1018516813604
发表时间：
1997-12-01
影响因子：
3.7
作者：
Huang, J;DiSilvio, L;Bonfield, W
通讯作者：
Bonfield, W