Common and distinctive genomic features of Klebsiella pneumoniae thriving in the natural environment or in clinical settings.
Common and distinctive genomic features of Klebsiella pneumoniae thriving in the natural environment or in clinical settings.
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在自然环境或临床环境中繁盛的肺炎克雷伯菌的共同和独特的基因组特征。
DOI：
10.1038/s41598-022-14547-6
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发表时间：
2022-06-21
影响因子：
4.6
通讯作者：
Manaia, Celia M.
中科院分区：
文献类型：
--
作者：
Rocha, Jaqueline;Henriques, Isabel;Gomila, Margarita;Manaia, Celia M.
肺炎克雷伯氏菌复合体由无处不在的细菌组成，可以在土壤、植物或水中发现，并作为人类的机会致病菌。本研究旨在推断临床和环境K.肺炎。K.肺炎克雷伯氏菌复合体（包括克雷伯氏菌）。variicola，n = 6;从GenBank中获得来自21个国家的临床来源（n = 78）和环境来源（n = 61）的类肺炎（n = 7）。这些基因组基于遗传学、泛基因组和选择的临床相关性状进行比较。基于核心基因组2704个基因的系统发育分析显示，临床菌株和环境菌株之间的亲缘关系密切，与多位点序列分型一致。在鉴定的62种序列类型（ST）中，有8种包括临床和环境基因组（ST 11、ST 14、ST 15、ST 37、ST 45、ST 147、ST 348、ST 437）。泛基因组关联研究并未证明临床和环境基因组之间存在显著差异。然而，临床分离株的基因组呈现出与抗生素耐药性/质粒相关的显著更多的排他性基因，而环境分离株产生了与功能（例如外排或氧化应激）相关的显著更高的等位基因多样性。研究表明，K.肺炎克雷伯氏菌可以在自然环境和临床环境中传播，可能是在不同的适应压力下。
The Klebsiella pneumoniae complex is comprised of ubiquitous bacteria that can be found in soils, plants or water, and as humans’ opportunistic pathogens. This study aimed at inferring common and distinctive features in clinical and environmental K. pneumoniae. Whole genome sequences of members of the K. pneumoniae complex (including K. variicola, n = 6; and K. quasipneumoniae, n = 7), of clinical (n = 78) and environmental (n = 61) origin from 21 countries were accessed from the GenBank. These genomes were compared based on phylogeny, pangenome and selected clinically relevant traits. Phylogenetic analysis based on 2704 genes of the core genome showed close relatedness between clinical and environmental strains, in agreement with the multi-locus sequence typing. Eight out of the 62 sequence types (STs) identified, included both clinical and environmental genomes (ST11, ST14, ST15, ST37, ST45, ST147, ST348, ST437). Pangenome-wide association studies did not evidence significant differences between clinical and environmental genomes. However, the genomes of clinical isolates presented significantly more exclusive genes related to antibiotic resistance/plasmids, while the environmental isolates yielded significantly higher allelic diversity of genes related with functions such as efflux or oxidative stress. The study suggests that K. pneumoniae can circulate among the natural environment and clinical settings, probably under distinct adaptation pressures.
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影响因子：
168.9
作者：
Holmes, Alison H.;Moore, Luke S. P.;Piddock, Laura J. V.
通讯作者：
Piddock, Laura J. V.
影响因子：
16.6
作者：
Lam MMC;Wick RR;Watts SC;Cerdeira LT;Wyres KL;Holt KE
通讯作者：
Holt KE
影响因子：
4.8
作者：
Long SW;Linson SE;Ojeda Saavedra M;Cantu C;Davis JJ;Brettin T;Olsen RJ
通讯作者：
Olsen RJ
影响因子：
6.4
作者：
Gomez-Simmonds, Angela;Uhlemann, Anne-Catrin
通讯作者：
Uhlemann, Anne-Catrin
过度抗呼吸和多药的肺炎克隆基团的基因组定义。
影响因子：
11.8
作者：
Bialek-Davenet S;Criscuolo A;Ailloud F;Passet V;Jones L;Delannoy-Vieillard AS;Garin B;Le Hello S;Arlet G;Nicolas-Chanoine MH;Decré D;Brisse S
通讯作者：
Brisse S