Genes and Gut Bacteria Involved in Luminal Butyrate Reduction Caused by Diet and Loperamide.
Genes and Gut Bacteria Involved in Luminal Butyrate Reduction Caused by Diet and Loperamide.
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DOI：
10.3390/genes8120350
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发表时间：
2017-11-28
期刊：
影响因子：
3.5
通讯作者：
Unno T
中科院分区：
文献类型：
--
作者：
Hwang N;Eom T;Gupta SK;Jeong SY;Jeong DY;Kim YS;Lee JH;Sadowsky MJ;Unno T
不平衡的饮食习惯和肠道动力障碍是代谢和功能性肠道疾病的致病因素，包括肥胖、糖尿病和便秘。众所周知，肠腔内丁酸合成的减少与肠道生物失调有关，研究表明，恢复结肠中丁酸的形成可能会改善肠道健康。相比之下，不同类型的肠道微生物群的变化可能会抑制管腔丁酸的合成，需要不同的治疗方法来恢复结肠细菌丁酸的合成。我们研究了高脂饮食(HFD)和低纤维饮食(LFD)以及洛哌丁胺(LPM)对小鼠体内关键细菌和参与减少丁酸合成的基因的影响。基于MiSeq的微生物区系分析和基于HiSeq的差异基因分析表明，在每个处理中都有不同类型的细菌和基因参与丁酸代谢。饮食调节通过减少类杆菌和副杆菌类的成员来耗尽丁酸激酶和磷酸丁酰转移酶。HFD还通过减少乳杆菌来耗尽与琥珀酸合成有关的基因。LFD和LPM处理通过减少Roseburia和Oscilllibacter来耗尽参与巴豆酰-CoA合成的基因。综上所述，我们的结果表明，不同类型的细菌和基因参与了肠道生物失调，可能需要根据肠道功能障碍的原因选择治疗方法。
Unbalanced dietary habits and gut dysmotility are causative factors in metabolic and functional gut disorders, including obesity, diabetes, and constipation. Reduction in luminal butyrate synthesis is known to be associated with gut dysbioses, and studies have suggested that restoring butyrate formation in the colon may improve gut health. In contrast, shifts in different types of gut microbiota may inhibit luminal butyrate synthesis, requiring different treatments to restore colonic bacterial butyrate synthesis. We investigated the influence of high-fat diets (HFD) and low-fiber diets (LFD), and loperamide (LPM) administration, on key bacteria and genes involved in reduction of butyrate synthesis in mice. MiSeq-based microbiota analysis and HiSeq-based differential gene analysis indicated that different types of bacteria and genes were involved in butyrate metabolism in each treatment. Dietary modulation depleted butyrate kinase and phosphate butyryl transferase by decreasing members of the Bacteroidales and Parabacteroides. The HFD also depleted genes involved in succinate synthesis by decreasing Lactobacillus. The LFD and LPM treatments depleted genes involved in crotonoyl-CoA synthesis by decreasing Roseburia and Oscilllibacter. Taken together, our results suggest that different types of bacteria and genes were involved in gut dysbiosis, and that selected treatments may be needed depending on the cause of gut dysfunction.
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影响因子：
3.7
作者：
Jakobsdottir G;Xu J;Molin G;Ahrné S;Nyman M
通讯作者：
Nyman M
影响因子：
3.7
作者：
Lecomte V;Kaakoush NO;Maloney CA;Raipuria M;Huinao KD;Mitchell HM;Morris MJ
通讯作者：
Morris MJ
影响因子：
14.9
作者：
Li PE;Lo CC;Anderson JJ;Davenport KW;Bishop-Lilly KA;Xu Y;Ahmed S;Feng S;Mokashi VP;Chain PS
通讯作者：
Chain PS
影响因子：
29
作者：
Donohoe DR;Garge N;Zhang X;Sun W;O'Connell TM;Bunger MK;Bultman SJ
通讯作者：
Bultman SJ
影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer