Changes in gut microbiota in rats fed a high fat diet correlate with obesity-associated metabolic parameters.
Changes in gut microbiota in rats fed a high fat diet correlate with obesity-associated metabolic parameters.
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DOI：
10.1371/journal.pone.0126931
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Morris MJ
中科院分区：
文献类型：
--
作者：
Lecomte V;Kaakoush NO;Maloney CA;Raipuria M;Huinao KD;Mitchell HM;Morris MJ
肠道微生物群正在成为肥胖发展的一个新因素。许多研究已经描述了在门水平上响应于肥胖和高脂肪饮食（HFD）的微生物群组成的变化。在这项研究中，我们对来自长期喂食HFD或对照食物的大鼠（每组n = 10，16周）的粪便样品使用16 s RNA高通量测序来研究物种水平上肠道微生物群组成的变化。在种的水平上，种群间的差异性为53.17%。乳酸杆菌在食物饮食下的大鼠中占主导地位。然而，在喂食HFD的大鼠中，该物种的丰度显著降低（P<0.0001），这通过丙酸盐/乙酸盐产生物种丰度的增加得到补偿。为了进一步了解这些物种对肥胖表型发展的影响，我们将它们的丰度与肥胖相关的代谢参数相关联。类群之间的差异贡献最大，10提出了显着的相关性与至少一个测试参数，其中三个正相关的所有代谢参数：Phascolarctobacterium，变形杆菌奇异和韦荣球菌，所有丙酸/乙酸生产商。乳酸杆菌是唯一的物种，其丰度与体重和脂肪量的变化呈负相关。该物种响应于HFD而急剧减少，有利于产生丙酸盐/乙酸盐的细菌物种，其丰度与肥胖和代谢因子的恶化密切相关。我们的观察结果表明，这些物种可能在HFD引起的肥胖发展中起关键作用。
The gut microbiota is emerging as a new factor in the development of obesity. Many studies have described changes in microbiota composition in response to obesity and high fat diet (HFD) at the phylum level. In this study we used 16s RNA high throughput sequencing on faecal samples from rats chronically fed HFD or control chow (n = 10 per group, 16 weeks) to investigate changes in gut microbiota composition at the species level. 53.17% dissimilarity between groups was observed at the species level. Lactobacillus intestinalis dominated the microbiota in rats under the chow diet. However this species was considerably less abundant in rats fed HFD (P<0.0001), this being compensated by an increase in abundance of propionate/acetate producing species. To further understand the influence of these species on the development of the obese phenotype, we correlated their abundance with metabolic parameters associated with obesity. Of the taxa contributing the most to dissimilarity between groups, 10 presented significant correlations with at least one of the tested parameters, three of them correlated positively with all metabolic parameters: Phascolarctobacterium, Proteus mirabilis and Veillonellaceae, all propionate/acetate producers. Lactobacillus intestinalis was the only species whose abundance was negatively correlated with change in body weight and fat mass. This species decreased drastically in response to HFD, favouring propionate/acetate producing bacterial species whose abundance was strongly correlated with adiposity and deterioration of metabolic factors. Our observations suggest that these species may play a key role in the development of obesity in response to a HFD.
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影响因子：
7.7
作者：
Cani, Patrice D.;Amar, Jacques;Burcelin, Remy
通讯作者：
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影响因子：
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DOI：
10.1152/ajpendo.00229.2010
发表时间：
2011-01-01
影响因子：
5.1
作者：
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通讯作者：
Bohlooly-Y, Mohammad