Direct-write bioprinting of cell-laden methacrylated gelatin hydrogels.
Direct-write bioprinting of cell-laden methacrylated gelatin hydrogels.
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DOI：
10.1088/1758-5082/6/2/024105
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发表时间：
2014-06
期刊：
影响因子：
9
通讯作者：
Khademhosseini A
中科院分区：
文献类型：
--
作者：
Bertassoni LE;Cardoso JC;Manoharan V;Cristino AL;Bhise NS;Araujo WA;Zorlutuna P;Vrana NE;Ghaemmaghami AM;Dokmeci MR;Khademhosseini A
具有可控微结构的三维(3D)有机化合物的制造已被证明可以增强组织功能。生物打印可以用来精确定位细胞和加载细胞的材料，以产生受控的组织结构。因此，它代表着一种令人兴奋的器官制造选择。尽管该领域取得了快速进展，但开发可用于从ECM水凝胶中制造大规模组织结构的打印工艺仍然是一个挑战。在这里，我们报告了一种策略，生物打印的光细胞负载甲基丙烯酸酯明胶(GelMA)水凝胶。我们以不同的细胞密度对负载细胞的GelMA进行了浓度从7%到15%的生物打印，发现印刷适性和水凝胶的机械性能之间存在直接的相关性。此外，微囊化的HepG2细胞在生物打印过程后至少保存了8天的细胞活力。综上所述，本工作提出了一种细胞载光ECM衍生水凝胶的直写生物打印策略，该策略可能会在组织工程、器官打印和3D药物发现平台的开发中得到广泛应用。
Fabrication of three dimensional (3D) organoids with controlled microarchitectures has been shown to enhance tissue functionality. Bioprinting can be used to precisely position cells and cell-laden materials to generate controlled tissue architecture. Therefore, it represents an exciting alternative for organ fabrication. Despite the rapid progress in the field, the development of printing processes that can be used to fabricate macroscale tissue constructs from ECM-derived hydrogels has remained a challenge. Here we report a strategy for bioprinting of photolabile cell-laden methacrylated gelatin (GelMA) hydrogels. We bioprinted cell-laden GelMA at concentrations ranging from 7 to 15% with varying cell densities and found a direct correlation between printability and the hydrogel mechanical properties. Furthermore, encapsulated HepG2 cells preserved cell viability for at least 8 days following the bioprinting process. In summary, this work presents a strategy for direct-write bioprinting of a cell-laden photolabile ECM-derived hydrogel, which may find widespread application for tissue engineering, organ printing and the development of 3D drug discovery platforms.
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影响因子：
29.4
作者：
Slaughter, Brandon V.;Khurshid, Shahana S.;Fisher, Omar Z.;Khademhosseini, Ali;Peppas, Nicholas A.
通讯作者：
Peppas, Nicholas A.
影响因子：
168.9
作者：
Raya-Rivera, Atlantida;Esquiliano, Diego R.;Yoo, James J.;Lopez-Bayghen, Esther;Soker, Shay;Atala, Anthony
通讯作者：
Atala, Anthony
影响因子：
5.5
作者：
Guillemot, Fabien;Souquet, Agnes;Guillotin, Bertrand
通讯作者：
Guillotin, Bertrand
影响因子：
6.1
作者：
Ramon-Azcon, Javier;Ahadian, Samad;Matsue, Tomokazu
通讯作者：
Matsue, Tomokazu
影响因子：
2.7
作者：
Carsin, H;Ainaud, P;Perrot, J
通讯作者：
Perrot, J