Prospecting sugarcane resistance to Sugarcane yellow leaf virus by genome-wide association.
Prospecting sugarcane resistance to Sugarcane yellow leaf virus by genome-wide association.
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DOI：
10.1007/s00122-014-2334-7
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发表时间：
2014-08
影响因子：
5.4
通讯作者：
Daugrois, J. H.
中科院分区：
文献类型：
--
作者：
Debibakas, S.;Rocher, S.;Garsmeur, O.;Toubi, L.;Roques, D.;D'Hont, A.;Hoarau, J. -Y.;Daugrois, J. H.
使用GWAS方法，我们在甘蔗中检测到了对载体病毒病的独立抗性标记。基于比较基因组学，确定了几个可能参与病毒/蚜虫/植物相互作用的候选基因。 甘蔗黄叶病是一种由蚜虫传播的甘蔗黄叶病毒（Sugarcane yellow leaf virus，SCYLV）引起的病毒性病害。为了确定数量性状基因座控制黄叶抗性育种直接相关，我们进行了全基因组关联研究（GWAS）的甘蔗品种面板（n = 189）代表目前的育种种质。用3，949个多态性标记（DArT和AFLP）对该组进行指纹分析。在瓜德罗普流行的自然疾病压力下，在两个作物周期的两个试验中，对该小组的叶和茎中的SCYLV感染进行表型分析。混合线性模型包括代表群体结构固定效应和成对亲缘关系随机效应的辅助因子，在全基因组水平上有效控制了I型错误的风险。检测到6个与SCYLV抗性表型显著相关的独立标记。这些标记分别解释了栽培品种组的9 - 14%的病害变异。他们在小组中的频率相对较低（8- 20%）。其中，基于不同的抗病性参数，在GWAS试验中重复检测到两个标记。这两个标记可以在高粱基因组上定位，并且可能参与植物-蚜虫或植物-病毒互作的候选基因定位在甘蔗标记的高粱同源物附近。我们的研究结果说明了GWAS方法在甘蔗种质资源中前景的潜力，以获得可能具有显著抗性等位基因的育种计划。本文的在线版本（doi：10.1007/s 00122 -014-2334-7）包含补充材料，可供授权用户使用。
Using GWAS approaches, we detected independent resistant markers in sugarcane towards a vectored virus disease. Based on comparative genomics, several candidate genes potentially involved in virus/aphid/plant interactions were pinpointed. Yellow leaf of sugarcane is an emerging viral disease whose causal agent is a Polerovirus, the Sugarcane yellow leaf virus (SCYLV) transmitted by aphids. To identify quantitative trait loci controlling resistance to yellow leaf which are of direct relevance for breeding, we undertook a genome-wide association study (GWAS) on a sugarcane cultivar panel (n = 189) representative of current breeding germplasm. This panel was fingerprinted with 3,949 polymorphic markers (DArT and AFLP). The panel was phenotyped for SCYLV infection in leaves and stalks in two trials for two crop cycles, under natural disease pressure prevalent in Guadeloupe. Mixed linear models including co-factors representing population structure fixed effects and pairwise kinship random effects provided an efficient control of the risk of inflated type-I error at a genome-wide level. Six independent markers were significantly detected in association with SCYLV resistance phenotype. These markers explained individually between 9 and 14 % of the disease variation of the cultivar panel. Their frequency in the panel was relatively low (8–20 %). Among them, two markers were detected repeatedly across the GWAS exercises based on the different disease resistance parameters. These two markers could be blasted on Sorghum bicolor genome and candidate genes potentially involved in plant–aphid or plant–virus interactions were localized in the vicinity of sorghum homologs of sugarcane markers. Our results illustrate the potential of GWAS approaches to prospect among sugarcane germplasm for accessions likely bearing resistance alleles of significant effect useful in breeding programs. The online version of this article (doi:10.1007/s00122-014-2334-7) contains supplementary material, which is available to authorized users.
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影响因子：
14.9
作者：
Bülow L;Schindler M;Hehl R
通讯作者：
Hehl R
影响因子：
11.6
作者：
Nalam, Vamsi J.;Keeretaweep, Jantana;Shah, Jyoti
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Shah, Jyoti
影响因子：
3.1
作者：
D'Hont, A;Ison, D;Glaszmann, JC
通讯作者：
Glaszmann, JC
影响因子：
1.9
作者：
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通讯作者：
Reynaud, B.
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影响因子：
5.3
作者：
Liu S;Han B
通讯作者：
Han B