SARS-CoV-2 Genomes From Oklahoma, United States.
SARS-CoV-2 Genomes From Oklahoma, United States.
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DOI：
10.3389/fgene.2020.612571
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发表时间：
2020
影响因子：
3.7
通讯作者：
Ramachandran A
中科院分区：
文献类型：
--
作者：
Narayanan S;Ritchey JC;Patil G;Narasaraju T;More S;Malayer J;Saliki J;Kaul A;Agarwal PK;Ramachandran A
基因组测序在了解严重急性呼吸综合征冠状病毒2 （SARS-CoV-2）的致病性方面发挥了重要作用。在当前大流行的情况下，必须定期对SARS-CoV-2病毒进行测序，以确定不同地理位置的突变和基因组修饰。在这项研究中，我们对在美国俄克拉荷马州大流行存在的不同时间点获得的5份临床样本进行了SARS-CoV-2测序。对来自该州大流行最初几天的一个样本和俄克拉荷马州高峰期的四个样本进行了测序。先前报道的突变包括S基因的D614G、ORF1ab基因的P4715L、N基因的S194L、R203K和G204R。在S基因（G1167V）、ORF1ab （A6269S和P3371S）、ORF7b （T28I）和ORF8 （G96R）中也检测到可能的新突变。基因组的系统发育分析显示与全球报告的其他SARS-CoV-2病毒相似。结构表征表明，S基因突变可能影响刺突蛋白的构象柔韧性和运动，N基因突变与核衣壳蛋白内连接子区紊乱有关。
Genomic sequencing has played a major role in understanding the pathogenicity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). With the current pandemic, it is essential that SARS-CoV-2 viruses are sequenced regularly to determine mutations and genomic modifications in different geographical locations. In this study, we sequenced SARS-CoV-2 from five clinical samples obtained in Oklahoma, United States during different time points of pandemic presence in the state. One sample from the initial days of the pandemic in the state and four during the peak in Oklahoma were sequenced. Previously reported mutations including D614G in S gene, P4715L in ORF1ab, S194L, R203K, and G204R in N gene were identified in the genomes sequenced in this study. Possible novel mutations were also detected in the S gene (G1167V), ORF1ab (A6269S and P3371S), ORF7b (T28I), and ORF8 (G96R). Phylogenetic analysis of the genomes showed similarity to other SARS-CoV-2 viruses reported from across the globe. Structural characterization indicates that the mutations in S gene possibly influences conformational flexibility and motion of the spike protein, and the mutations in N gene are associated with disordered linker region within the nucleocapsid protein.
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