Stretch activation of a toad smooth muscle K+ channel may be mediated by fatty acids.
Stretch activation of a toad smooth muscle K+ channel may be mediated by fatty acids.
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蟾蜍平滑肌 K 通道的伸展激活可能是由脂肪酸介导的。
DOI：
10.1113/jphysiol.1995.sp020668
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Singer,JJ
中科院分区：
文献类型：
--
作者：
Ordway,RW;Petrou,S;Kirber,MT;WalshJr,JV;Singer,JJ
1.使用标准的单通道膜片钳技术，我们研究了20 pS的K（+）选择性通道的牵张敏感性，该通道被脂肪酸激活，并在来自蟾蜍Bufo marinus胃的新鲜分离的平滑肌细胞中发现。2.为了拉伸膜，在贴片移液管的背面施加抽吸脉冲，导致该K+通道的激活。一系列抽吸脉冲导致每个连续脉冲期间通道活动水平逐渐增加，以及脉冲之间基线活动增加。这种模式与许多其他拉伸激活通道形成鲜明对比，这些通道的激活仅限于抽吸脉冲的持续时间。3.脂肪酸的应用增强了对拉伸的反应。相比之下，应用10 μ M脱脂白蛋白，从膜中除去脂肪酸，迅速和可逆地降低了对拉伸的反应。4.这些结果是一致的假设，即脂肪酸产生的机械刺激，也许是机械激活磷脂酶，是在激活某些机械敏感的离子通道的中介。
1. using standard single channel patch clamp techniques we studied the stretch sensitivity of a 20 pS K(+)‐selective channel which is activated by fatty acids and found in freshly dissociated smooth muscle cells from the stomach of the toad Bufo marinus. 2. A pulse of suction applied to the back of the patch pipette in order to stretch the membrane resulted in activation of this K+ channel. A train of suction pulses resulted in a gradually increased level of channel activity during each successive pulse, as well as an increase in baseline activity between pulses. This pattern contrasts markedly with many other stretch‐activated channels whose activation is limited to the duration of the suction pulse. 3. Application of fatty acids augmented the response to stretch. In contrast, application of 10 microM defatted albumin, which removes fatty acids from membranes, rapidly and reversibly decreased the response to stretch. 4. These results are consistent with the hypothesis that fatty acids which are generated by mechanical stimuli, perhaps by mechanically activated phospholipases, are the intermediaries in activation of certain mechanically sensitive ion channels.
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影响因子：
56.9
作者：
ORDWAY, RW;WALSH, JV;SINGER, JJ
通讯作者：
SINGER, JJ
FMRFamide 和膜拉伸作为海兔 S 通道的激活剂。
影响因子：
3.4
作者：
David H. Vandorpe;Daniel L. Small;André Robert Dabrowski;Catherine E. Morris
通讯作者：
Catherine E. Morris
通过底物单层的“垂直压缩”控制磷脂酶 A 的作用。
影响因子：
2.9
作者：
T. Thuren;J. Virtanen;P. Kinnunen
通讯作者：
P. Kinnunen
拉伸敏感离子通道：更新。
DOI：
--
发表时间：
1992
期刊：
Society of General Physiologists series
影响因子：
--
作者：
Sachs,F
通讯作者：
Sachs,F
DOI：
10.1073/pnas.89.23.11367
发表时间：
1992-12-01
影响因子：
11.1
作者：
KAMP, F;HAMILTON, JA
通讯作者：
HAMILTON, JA