Identification of a novel Gig2 gene family specific to non-amniote vertebrates.
Identification of a novel Gig2 gene family specific to non-amniote vertebrates.
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非羊膜脊椎动物特有的新 Gig2 基因家族的鉴定
DOI：
10.1371/journal.pone.0060588
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Gui JF
中科院分区：
文献类型：
--
作者：
Zhang YB;Liu TK;Jiang J;Shi J;Liu Y;Li S;Gui JF
Gig 2（grasscarpreovirusGCRV-inducedgene 2）是首次发现的一个新的鱼类干扰素刺激基因（ISG）。斑马鱼Gig 2基因的过表达可以保护培养的鱼细胞免受病毒感染。在本研究中，我们确定了一个新的基因家族，该家族由与先前表征的Gig 2同源的基因组成。EST/GSS搜索和电子克隆鉴定了从七鳃鳗到两栖动物的51种脊椎动物中的190个Gig 2同源基因。对脊椎动物和无脊椎动物基因组数据库的进一步大规模检索表明，Gig 2基因家族是包括七鳃鳗、鲨鱼/鳐、鳍鱼和两栖动物在内的非脊椎动物特有的。系统发育分析和共线性分析揭示了Gig 2基因家族的谱系特异性扩展，也为鱼类特异性基因组复制（FSGD）假说提供了有价值的证据。尽管Gig 2家族蛋白与任何已知蛋白没有显著的序列相似性，但典型的Gig 2蛋白似乎由两个保守部分组成：与聚（ADP-核糖）聚合酶（PARP）的催化结构域具有非常低的同源性的N-末端，以及该基因家族所特有的新的C-末端结构域。斑马鱼Gig 2家族基因的表达谱分析表明，一些重复的对已经分歧的功能，通过收购新的空间和/或时间的表达压力。该基因家族对非羊水动物的特异性可能在很大程度上导致了非羊水脊椎动物的独特生理学。
Gig2 (grass carp reovirus (GCRV)-induced gene 2) is first identified as a novel fish interferon (IFN)-stimulated gene (ISG). Overexpression of a zebrafish Gig2 gene can protect cultured fish cells from virus infection. In the present study, we identify a novel gene family that is comprised of genes homologous to the previously characterized Gig2. EST/GSS search and in silico cloning identify 190 Gig2 homologous genes in 51 vertebrate species ranged from lampreys to amphibians. Further large-scale search of vertebrate and invertebrate genome databases indicate that Gig2 gene family is specific to non-amniotes including lampreys, sharks/rays, ray-finned fishes and amphibians. Phylogenetic analysis and synteny analysis reveal lineage-specific expansion of Gig2 gene family and also provide valuable evidence for the fish-specific genome duplication (FSGD) hypothesis. Although Gig2 family proteins exhibit no significant sequence similarity to any known proteins, a typical Gig2 protein appears to consist of two conserved parts: an N-terminus that bears very low homology to the catalytic domains of poly(ADP-ribose) polymerases (PARPs), and a novel C-terminal domain that is unique to this gene family. Expression profiling of zebrafish Gig2 family genes shows that some duplicate pairs have diverged in function via acquisition of novel spatial and/or temporal expression under stresses. The specificity of this gene family to non-amniotes might contribute to a large extent to distinct physiology in non-amniote vertebrates.
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