Role of genetic susceptibility to latent adenoviral infection and decreased lung function.
Role of genetic susceptibility to latent adenoviral infection and decreased lung function.
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DOI：
10.1016/j.rmed.2009.05.008
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发表时间：
2009-11
影响因子：
4.3
通讯作者：
Sandford, Andrew J.
中科院分区：
文献类型：
--
作者：
Kasuga, Ikuma;Hogg, James C.;Pare, Peter D.;Hayashi, Shizu;Sedgwick, Edward G.;Ruan, Jian;Wallace, Alison M.;He, Jian-Qing;Zhang, Xiaozhu;Sandford, Andrew J.
潜伏性腺病毒感染可加重香烟烟雾诱导的肺部炎症，因此在慢性阻塞性肺疾病（COPD）的发展中起重要作用。腺病毒可以通过其19-kDa蛋白（19 K）逃避人类免疫应答，所述19-kDa蛋白（19 K）延迟I类人类白细胞抗原（HLA）蛋白的表达。与HLA-A1和A3相比，19 K蛋白对HLA-B7和A2显示出更高的亲和力。腺病毒受体（CXADR）和整合素β5（ITGB 5）是影响腺病毒感染的宿主因子。因此，我们研究了HLA、CXADR和ITGB 5遗传变异对E1 A基因存在和肺功能水平的贡献。使用等位基因特异性引物，通过基于PCR的测定法测定研究受试者的HLA-B7、A1、A2和A3。CXADR和ITGB 5基因的多态性通过基于PCR的限制性片段长度多态性分析进行基因分型。采用实时荧光定量PCR TaqMan法检测腺病毒E1 A基因。E1 A阳性者FEV 1低于E1 A阴性者。而19 K高亲和力组（HLA-B7和A2）与低亲和力组（HLA-A1和A3）E1 A阳性率无显著性差异。FEV 1水平在各亲和组之间也无显著差异。CXADR和ITGB 5基因型间E1 A阳性率和肺功能无显著性差异。HLA、CXADR和ITGB 5的遗传变异不影响潜伏性腺病毒感染，与COPD无关。
Latent adenoviral infection may amplify cigarette smoke-induced lung inflammation and therefore play an important role in the development of chronic obstructive pulmonary disease (COPD). Adenoviruses can evade the human immune response via their 19-kDa protein (19K) which delays the expression of class I human leukocyte antigen (HLA) proteins. The 19K protein shows higher affinity to HLA-B7 and A2 compared with HLA-A1 and A3. The receptor for adenovirus (CXADR) and integrin β5 (ITGB5) are host factors which might affect adenovirus infection. Therefore, we investigated the contribution of HLA, CXADR, and ITGB5 genetic variants to the presence of the E1A gene and to level of lung function. Study subjects were assayed for HLA-B7, A1, A2 and A3 by PCR-based assays using allele-specific primers. Polymorphisms of the CXADR and ITGB5 genes were genotyped by PCR-based restriction fragment length polymorphism assays. Detection of adenoviral E1A gene was performed by a real-time PCR TaqMan assay. E1A positive individuals have a lower FEV1 compared with E1A negative individuals. However, there was no significant difference in E1A positivity rate between the high (HLA-B7 and A2) and low (HLA-A1 and A3) 19K affinity groups. There was also no significant difference in FEV1 level between each affinity group. There was no significant difference in E1A positivity rate or lung function among the CXADR and ITGB5 genotypes. Genetic variants in HLA, CXADR and ITGB5 do not influence latent adenoviral infections and are not associated with COPD.
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影响因子：
5.8
作者：
Bergelson, JM
通讯作者：
Bergelson, JM
影响因子：
105.7
作者：
FLETCHER, C;PETO, R
通讯作者：
PETO, R
影响因子：
64.5
作者：
ANDERSSON, M;PAABO, S;PETERSON, PA
通讯作者：
PETERSON, PA
影响因子：
--
作者：
BLASCZYK, R;HAHN, U;SALAMA, A
通讯作者：
SALAMA, A
DOI：
10.1073/pnas.76.12.6606
发表时间：
1979-01-01
影响因子：
11.1
作者：
GREEN, M;WOLD, WSM;RIGDEN, P
通讯作者：
RIGDEN, P