Involvement of membrane palmitoylated protein 2 (MPP2) in the synaptic molecular complex at the mouse cerebellar glomerulus
Involvement of membrane palmitoylated protein 2 (MPP2) in the synaptic molecular complex at the mouse cerebellar glomerulus
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膜棕榈酰化蛋白 2 (MPP2) 参与小鼠小脑肾小球突触分子复合物
DOI：
10.1007/s00418-022-02137-6
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发表时间：
2022
影响因子：
2.3
通讯作者：
Nobuo Terada
中科院分区：
文献类型：
--
作者：
Tomoki Yamada;Yurika Saitoh;Kiyokazu Kametani;Akio Kamijo;Takeharu Sakamoto;Nobuo Terada
我们先前报道了外周神经系统中的膜骨架蛋白4.1G转运膜棕榈酰化蛋白6（MPP 6），MPP 6与形成髓鞘的许旺细胞中的突触支架蛋白Lin 7和细胞粘附分子4（CADM 4）相互作用。在本研究中，我们研究了MPP 2，MPP 6的高度同源的家族蛋白，在小鼠中枢神经系统的小脑，其中神经元组织良好的定位和相关蛋白。出生后第14天，在小脑肾小球（CG）的颗粒层中观察到MPP 2的免疫染色。使用共聚焦激光扫描显微镜的高分辨率Airyscan模式，MPP 2被检测为点图案，并与CADM 1和Lin 7共定位，被识别为小环/线图案，以及与钙/钙调蛋白依赖性丝氨酸蛋白激酶（CASK）、NMDA谷氨酸受体1（GluN 1）和M-钙粘蛋白共定位，被识别为点图案，提示MPP 2定位于兴奋性突触后区和颗粒细胞的粘附连接处。免疫沉淀分析显示，MPP 2与CADM 1、CASK、M-cadherin和Lin 7形成分子复合物。此外，在野生型CG中，Lin 7染色模式显示围绕苔藓纤维的小环，而在MPP 2缺陷型CG中，其改变为CADM 1染色检测到的小环内的点/点模式。这些结果表明，MPP 2的影响，在CG，在电信号进入小脑的颗粒细胞的突触后区域的突触细胞膜上的Lin 7的分布。
We previously reported that the membrane skeletal protein 4.1G in the peripheral nervous system transports membrane palmitoylated protein 6 (MPP6), which interacts with the synaptic scaffolding protein Lin7 and cell adhesion molecule 4 (CADM4) in Schwann cells that form myelin. In the present study, we investigated the localization of and proteins related to MPP2, a highly homologous family protein of MPP6, in the cerebellum of the mouse central nervous system, in which neurons are well organized. Immunostaining for MPP2 was observed at cerebellar glomeruli (CG) in the granular layer after postnatal day 14. Using the high-resolution Airyscan mode of a confocal laser-scanning microscope, MPP2 was detected as a dot pattern and colocalized with CADM1 and Lin7, recognized as small ring/line patterns, as well as with calcium/calmodulin-dependent serine protein kinase (CASK), NMDA glutamate receptor 1 (GluN1), and M-cadherin, recognized as dot patterns, indicating the localization of MPP2 in the excitatory postsynaptic region and adherens junctions of granule cells. An immunoprecipitation analysis revealed that MPP2 formed a molecular complex with CADM1, CASK, M-cadherin, and Lin7. Furthermore, the Lin7 staining pattern showed small rings surrounding mossy fibers in wild-type CG, while it changed to the dot/spot pattern inside small rings detected with CADM1 staining in MPP2-deficient CG. These results indicate that MPP2 influences the distribution of Lin7 to synaptic cell membranes at postsynaptic regions in granule cells at CG, at which electric signals enter the cerebellum.
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DOI：
10.3390/molecules25214954
发表时间：
2020-10-26
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Chytła A;Gajdzik-Nowak W;Olszewska P;Biernatowska A;Sikorski AF;Czogalla A
通讯作者：
Czogalla A
DOI：
10.1083/jcb.121.3.491
发表时间：
1993-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Itoh M;Nagafuchi A;Yonemura S;Kitani-Yasuda T;Tsukita S;Tsukita S
通讯作者：
Tsukita S
NMDA 受体亚基 GluRepsilon1、GluRepsilon3 和 GluRzeta1 在成年小鼠小脑的苔藓纤维颗粒细胞突触处富集。
影响因子：
3.4
作者：
K. Yamada;M. Fukaya;H. Shimizu;K. Sakimura;M. Watanabe
通讯作者：
M. Watanabe
DOI：
10.1073/pnas.88.18.8024
发表时间：
1991-09-01
影响因子：
11.1
作者：
DONALIES, M;CRAMER, M;STARZINSKIPOWITZ, A
通讯作者：
STARZINSKIPOWITZ, A
影响因子：
3.3
作者：
Heeyoun Park;Yukio Yamamoto;Keiko Tanaka-Yamamoto
通讯作者：
Heeyoun Park;Yukio Yamamoto;Keiko Tanaka-Yamamoto