Biorefining Potential of Wild-Grown Arundo donax, Cortaderia selloana and Phragmites australis and the Feasibility of White-Rot Fungi-Mediated Pretreatments.
Biorefining Potential of Wild-Grown Arundo donax, Cortaderia selloana and Phragmites australis and the Feasibility of White-Rot Fungi-Mediated Pretreatments.
复制标题
DOI：
10.3389/fpls.2021.679966
复制
发表时间：
2021
影响因子：
5.6
通讯作者：
Canhoto JM
中科院分区：
文献类型：
--
作者：
da Costa RMF;Winters A;Hauck B;Martín D;Bosch M;Simister R;Gomez LD;Batista de Carvalho LAE;Canhoto JM
黄竹藤（Arundo donax）、黄竹藤（Cortaderia selloana）和芦苇（Phragmites australis）是多年生高生物量的Poalean物种，在温暖的温带地区大量自然生长，如地中海型气候，如南欧、美国西部沿海地区，或南美洲、南非和澳大利亚地区。由于这些禾草的生长旺盛和自发，它们的生物量往往在不受管理的农林业地区过度积累。尽管如此，这也为这些生物质资源的价值增值创造了需求和机会，特别是它们的细胞壁聚合物，用于生物精炼应用。相比之下，相关作物芒草（Miscanthus x giganteus）是一种多年生草，由于它可以在较冷气候下的低投入农业系统中生长，因此已被广泛研究用于木质纤维素生物质生产。本研究采用傅里叶变换中红外光谱（FTIR）、高效阴离子交换色谱（HPAEC）和木质素含量测定方法，比较了野外收获的donax、C. selloana和P. australis的组成特征，并与田间种植的M. x giganteus高产基因型进行了比较。高通量糖化实验表明，即使在0.1M NaOH温和碱预处理下，野生草的糖释放值也相对较高。除了碱性预处理外，生物质还用白腐菌（WRF）处理，它比全息纤维素更容易降解木质素。选用三种真菌：灵芝、平菇和色曲霉。结果表明，在预处理过程中，中性糖含量没有明显变化，但木质素有一定损失。此外，酶促糖化过程中糖的释放增强，这取决于处理中使用的植物生物量和真菌种类。为了最大限度地发挥木质纤维素增值的潜力，通过高效液相色谱-光电二极管阵列检测-电喷雾电离串联质谱（HPLC-PDA-ESI-MSn）对预处理后的液体组分进行分析。这项研究是第一个报道wrf处理的草生物量组成的研究之一，同时评估了处理过程中释放的分解产物的潜在相关性，而不仅仅是传统的糖换能应用。最终，我们期望我们的数据将有助于促进未使用生物质资源的增值，创造经济价值，同时为可持续生物精炼系统的实施做出贡献。
Arundo donax, Cortaderia selloana and Phragmites australis are high-biomass-producing perennial Poalean species that grow abundantly and spontaneously in warm temperate regions, such as in Mediterranean-type climates, like those of Southern Europe, Western United States coastal areas, or in regions of South America, South Africa and Australia. Given their vigorous and spontaneous growth, biomass from the studied grasses often accumulates excessively in unmanaged agro-forestry areas. Nonetheless, this also creates the demand and opportunity for the valorisation of these biomass sources, particularly their cell wall polymers, for biorefining applications. By contrast, a related crop, Miscanthus × giganteus, is a perennial grass that has been extensively studied for lignocellulosic biomass production, as it can grow on low-input agricultural systems in colder climates. In this study Fourier transform mid-infrared spectroscopy (FTIR), high-performance anion-exchange chromatography (HPAEC) and lignin content determinations were used for a comparative compositional characterisation of A. donax, C. selloana and P. australis harvested from the wild, in relation to a trial field-grown M. × giganteus high-yielding genotype. A high-throughput saccharification assay showed relatively high sugar release values from the wild-grown grasses, even with a 0.1M NaOH mild alkali pretreatment. In addition to this alkaline pretreatment, biomass was treated with white-rot fungi (WRF), which preferentially degrade lignin more readily than holocellulose. Three fungal species were used: Ganoderma lucidum, Pleurotus ostreatus and Trametes versicolor. Our results showed that neutral sugar contents are not significantly altered, while some lignin is lost during the pretreatments. Furthermore, sugar release upon enzymatic saccharification was enhanced, and this was dependent on the plant biomass and fungal species used in the treatment. To maximise the potential for lignocellulose valorisation, the liquid fractions from the pretreatments were analysed by high performance liquid chromatography – photodiode array detection – electrospray ionisation tandem mass spectrometry (HPLC-PDA-ESI-MSn). This study is one of the first to report on the composition of WRF-treated grass biomass, while assessing the potential relevance of breakdown products released during the treatments, beyond more traditional sugar-for-energy applications. Ultimately, we expect that our data will help promote the valorisation of unused biomass resources, create economic value, while contributing to the implementation of sustainable biorefining systems.
登录
查看更多内容
影响因子：
4.2
作者：
da Costa RM;Lee SJ;Allison GG;Hazen SP;Winters A;Bosch M
通讯作者：
Bosch M
影响因子：
4.2
作者：
da Costa RMF;Simister R;Roberts LA;Timms-Taravella E;Cambler AB;Corke FMK;Han J;Ward RJ;Buckeridge MS;Gomez LD;Bosch M
通讯作者：
Bosch M
影响因子：
11.4
作者：
Campbell, J. Elliott;Lobell, David B.;Field, Christopher B.
通讯作者：
Field, Christopher B.
DOI：
10.1007/978-1-0716-0621-6_16
发表时间：
2020-01-01
期刊：
PLANT CELL WALL, 2 EDITION
影响因子：
--
作者：
da Costa, Ricardo M. F.;Barrett, William;Allison, Gordon G.
通讯作者：
Allison, Gordon G.
DOI：
10.1111/nph.14306
发表时间：
2017-03
期刊：
The New phytologist
影响因子：
--
作者：
da Costa RM;Pattathil S;Avci U;Lee SJ;Hazen SP;Winters A;Hahn MG;Bosch M
通讯作者：
Bosch M