Rapid functional divergence of a newly evolved polyubiquitin gene in Drosophila and its role in the trade-off between male fecundity and lifespan.
Rapid functional divergence of a newly evolved polyubiquitin gene in Drosophila and its role in the trade-off between male fecundity and lifespan.
复制标题
果蝇中新进化的多聚泛素基因的快速功能分化及其在雄性繁殖力和寿命之间权衡中的作用。
DOI：
10.1093/molbev/msr299
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发表时间：
2012-05
期刊：
影响因子：
--
通讯作者：
Wang W
中科院分区：
文献类型：
--
作者：
Zhan Z;Ding Y;Zhang Y;Yang S;Yu H;Zhou Q;Zhao R;Xiang H;Wang W
繁殖成本是生物进化过程中各种生物过程的关键权衡。然而，平衡生殖能力及其成本的进化背后的基因和分子机制在很大程度上仍然是未知的。本文对一对新进化的X连锁多聚泛素串联重复基因CG32744和CG11700的进化、表达和生物学功能进行了全面的研究。模拟进化枝我们发现，CG32744保留了果蝇物种中保守的多聚泛素编码序列，并普遍表达，而CG11700积累了许多氨基酸的变化，并显示出男性特异性表达模式。CG11700的突变体具有较高的雄性生殖力，但寿命较短，而其过表达降低雄性生殖力。相反，肽保守的CG32744的无效突变体不表现出这样的表型。这些结果表明，CG11700可能经历了新功能化，并在雄性生殖力和寿命之间的权衡中进化出重要的功能，CG32744可能保留了祖先的功能。
The cost of reproduction is a pivotal trade-off with various biological processes during the evolution of organisms. However, the genes and molecular mechanisms underlying the evolution of balancing reproductive capacity and its cost are still largely unknown. Here, we present a comprehensive study on the evolution, expression, and biological functions of a newly evolved pair of X-linked polyubiquitin tandemly duplicated genes, CG32744 and CG11700, of which the duplication event occurred in Drosophila melanogaster lineage after the split from D. simulans clade. We found that CG32744 retains conserved polyubiquitin-coding sequences across Drosophila species and is ubiquitously expressed, whereas CG11700 has accumulated numerous amino acid changes and shows a male-specific expression pattern. Null mutants of CG11700 have a higher male fecundity but shorter lifespan, whereas its overexpression decreases male fecundity. In contrast, the null mutants of the peptide-conserved CG32744 do not exhibit such phenotypes. These results suggest that CG11700 might have experienced neofunctionalization and evolved important functions in the trade-off between male fecundity and lifespan and that CG32744 likely has retained the ancestral function.
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影响因子：
4.5
作者：
Ding Y;Zhao L;Yang S;Jiang Y;Chen Y;Zhao R;Zhang Y;Zhang G;Dong Y;Yu H;Zhou Q;Wang W
通讯作者：
Wang W
DOI：
10.1038/nrg2487
发表时间：
2009-01
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
--
关于果蝇新基因的起源。
影响因子：
7
作者：
Zhou, Qi;Zhang, Guojie;Wang, Wen
通讯作者：
Wang, Wen
影响因子：
10.7
作者：
S. Sawyer
通讯作者：
S. Sawyer
影响因子：
10.7
作者：
Meisel, Richard P.;Hilldorfer, Benedict B.;Schaeffer, Stephen W.
通讯作者：
Schaeffer, Stephen W.