Wavelength Dependence of the Fluorescence Quenching Efficiency of Nearby Dyes by Gold Nanoclusters and Nanoparticles: The Roles of Spectral Overlap and Particle Size.
Wavelength Dependence of the Fluorescence Quenching Efficiency of Nearby Dyes by Gold Nanoclusters and Nanoparticles: The Roles of Spectral Overlap and Particle Size.
复制标题
DOI：
10.1021/jp204836w
复制
发表时间：
2011
影响因子：
3.7
通讯作者：
Peteanu, Linda A.
中科院分区：
文献类型：
--
作者：
Chowdhury, Sanchari;Wu, Zhikun;Jaquins-Gerstl, Andrea;Liu, Shengpeng;Dembska, Anna;Armitage, Bruce A.;Jin, Rongchao;Peteanu, Linda A.
将谷胱甘肽单层保护的金纳米簇（NC）AU25（1.2 nm金属核直径（D））与2和4 nm金纳米颗粒（NPS）相比，将染料插入DNA的染料的发射液的效率。在所有情况下，DNA/染料部分和金颗粒不是共价附着的，而是形成非共价基态络合物。在这些条件下，稳态测量结果表明，Au25的淬火效率比等离子4 nm金NP的淬火效率低10倍，但与未显示不同等离子体带的2 nm颗粒相当。但是，即使在非常低（NM）的Au25浓度下，也观察到明显的排放淬火。对于在等离子体峰的波长附近排放的染料，4 nm NP的淬灭效率明显更高，而2 nm金NP的染料由纳米表面能传递（NSET）模型很好地描述了Strouse组（J. Am。Chem。Chem。127，3115 2005）。有趣的是，对于Au25，对于在与2和4 nm NP相同的波长范围内发射的染料（490-560 nm）中发出的最大淬灭效率发生，在那里它显示没有离散的吸收特征，而不是与其同时发生的波长与其同性恋，内部lumo，内部频段，频段或频段间或频段间过渡。因此，发现AU25 NC的荧光淬灭特性与较大的NC和NP的荧光特性不同，但似乎不符合迄今为止提出的理论预测。
The efficiency of the glutathione monolayer-protected gold nanocluster (NC) Au25 (1.2 nm metal core diameter (d)) in quenching the emission of dyes intercalated into DNA is compared to that of 2 and 4 nm gold nanoparticles (NPs). In all cases, the DNA/dye moieties and the gold particles are not covalently attached but rather form non-covalent ground state complexes. Under these conditions, steady-state measurements reveal that the quenching efficiency of Au25 is a factor of 10 lower than that of plasmonic 4 nm gold NPs but comparable to that of 2 nm particles which do not show a distinct plasmon band. Nonetheless, significant emission quenching is observed even at very low (nM) concentrations of Au25. The quenching efficiency of the 4 nm NPs is significantly higher for dyes emitting near the wavelength of the plasmon peak whereas that of the 2 nm gold NPs is well described by the nano-surface energy transfer (NSET) model proposed by the Strouse group (J. Am. Chem. Soc. 127, 3115 2005). Interestingly, for Au25 the maximum quenching efficiency occurs for dyes emitting in the same wavelength range as that of the 2 and 4 nm NPs (490-560 nm), where it shows no discrete absorption features, rather than for wavelengths coincident with its HOMO-LUMO, intra-band or inter-band transitions. The fluorescence quenching properties of Au25 NCs are therefore found to be distinct from those of larger NCs and NPs but do not appear to conform to theoretical predictions advanced thus far.
登录
查看更多内容
影响因子：
14.9
作者：
Günther K;Mertig M;Seidel R
通讯作者：
Seidel R
影响因子：
46.9
作者：
Dubertret, B;Calame, M;Libchaber, AJ
通讯作者：
Libchaber, AJ
DOI：
10.1073/pnas.1132025100
发表时间：
2003-05-27
影响因子：
11.1
作者：
Fan, CH;Wang, S;Heeger, AJ
通讯作者：
Heeger, AJ
影响因子：
3.7
作者：
Lu, Yin;Dasog, Mita;Paige, Matthew F.
通讯作者：
Paige, Matthew F.
影响因子：
64.8
作者：
GLAZER, AN;RYE, HS
通讯作者：
RYE, HS