ADP1 affects plant architecture by regulating local auxin biosynthesis.
ADP1 affects plant architecture by regulating local auxin biosynthesis.
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ADP1 通过调节局部生长素生物合成影响植物结构
DOI：
10.1371/journal.pgen.1003954
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发表时间：
2014-01
期刊：
影响因子：
4.5
通讯作者：
Qu LJ
中科院分区：
文献类型：
--
作者：
Li R;Li J;Li S;Qin G;Novák O;Pěnčík A;Ljung K;Aoyama T;Liu J;Murphy A;Gu H;Tsuge T;Qu LJ
植物结构是影响植物生存和生产力的关键因素之一。植物的体细胞结构是在胚胎发生后，由顶端分生组织和根尖分生组织分化而来的侧生器官反复发生和生长而形成的。在这里，我们报道了ADP1，一个可能的配对(多药和有毒化合物挤出)转运体，在调节侧枝器官的生长，从而维持拟南芥的正常结构方面发挥着重要作用。ADP1表达水平的提高导致植株生长速度加快，腋枝数和花朵数增加。我们的分子和遗传证据表明，过量表达ADP1的植物的表型是由于分生组织区域局部生长素水平的降低造成的。我们进一步发现，根据测量的IAA水平和基因表达数据，这种下降可能是由于局部分生区生长素生物合成水平下降所致。同时失活ADP1及其最接近的三个同源物会导致生长迟缓、侧部器官数量相对减少和生长素水平略有升高。我们的结果表明，ADP1介导的对分生区局部生长素水平的调节是通过抑制侧生器官的生长来维持植物结构的一个重要决定因素。
Plant architecture is one of the key factors that affect plant survival and productivity. Plant body structure is established through the iterative initiation and outgrowth of lateral organs, which are derived from the shoot apical meristem and root apical meristem, after embryogenesis. Here we report that ADP1, a putative MATE (multidrug and toxic compound extrusion) transporter, plays an essential role in regulating lateral organ outgrowth, and thus in maintaining normal architecture of Arabidopsis. Elevated expression levels of ADP1 resulted in accelerated plant growth rate, and increased the numbers of axillary branches and flowers. Our molecular and genetic evidence demonstrated that the phenotypes of plants over-expressing ADP1 were caused by reduction of local auxin levels in the meristematic regions. We further discovered that this reduction was probably due to decreased levels of auxin biosynthesis in the local meristematic regions based on the measured reduction in IAA levels and the gene expression data. Simultaneous inactivation of ADP1 and its three closest homologs led to growth retardation, relative reduction of lateral organ number and slightly elevated auxin level. Our results indicated that ADP1-mediated regulation of the local auxin level in meristematic regions is an essential determinant for plant architecture maintenance by restraining the outgrowth of lateral organs.
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影响因子：
9.2
作者：
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通讯作者：
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