Assembly of the fluorescent acrosomal matrix and its fate in fertilization in the water strider, Aquarius remigis.
Assembly of the fluorescent acrosomal matrix and its fate in fertilization in the water strider, Aquarius remigis.
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DOI：
10.1002/jcp.22413
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发表时间：
2011-04
影响因子：
5.6
通讯作者：
Cardullo, Richard A.
中科院分区：
文献类型：
--
作者：
Miyata, Haruhiko;Noda, Naoki;Fairbairn, Daphne J.;Oldenbourg, Rudolf;Cardullo, Richard A.
动物精子在形态和分子组成上表现出显着的多样性。在这里，我们首次报告了动物精子中强烈的内在荧光。来自半水生昆虫水黾 (Aquarius remigis) 的精子含有一种本质荧光分子，其特性与黄素腺嘌呤二核苷酸 (FAD) 一致，黄素腺嘌呤二核苷酸 (FAD) 首先出现在圆形精子细胞的顶体囊泡中，并在整个精子发生过程中持续存在于顶体中。光漂白后的荧光恢复表明，荧光分子在圆形精子细胞的顶体囊泡中表现出不受限制的活动性，但在成熟精子的顶体中则完全不活动。荧光偏振显微镜显示成熟精子顶体中的荧光分子呈净排列，但圆形精子细胞的顶体囊泡中却没有。这些结果表明顶体分子在伸长的顶体中重新排列，并且 FAD 在顶体形成过程中并入顶体基质中。此外，我们利用内在荧光跟踪了顶体基质在受精过程中的命运。在受精卵内部观察到荧光顶体基质，即使在原肠胚形成后，顶体基质仍保持结构完整。这一观察结果表明，FAD 在受精过程或早期发育过程中不会从顶体基质中释放，并支持 FAD 参与顶体基质形成的观点。 A. remigis 顶体的内在荧光将成为后续精子发生和受精的有用标记。
Animal sperm show remarkable diversity in both morphology and molecular composition. Here we provide the first report of intense intrinsic fluorescence in an animal sperm. The sperm from a semi-aquatic insect, the water strider, Aquarius remigis, contains an intrinsically fluorescent molecule with properties consistent with those of Flavin Adenine Dinucleotid (FAD) which appears first in the acrosomal vesicle of round spermatids and persists in the acrosome throughout spermiogenesis. Fluorescence recovery after photobleaching reveals that the fluorescent molecule exhibits unrestricted mobility in the acrosomal vesicle of round spermatids, but is completely immobile in the acrosome of mature sperm. Fluorescence polarization microscopy shows a net alignment of the fluorescent molecules in the acrosome of the mature sperm but not in the acrosomal vesicle of round spermatids. These results suggest that acrosomal molecules are rearranged in the elongating acrosome and FAD is incorporated into the acrosomal matrix during its formation. Further, we followed the fate of the acrosomal matrix in fertilization utilizing the intrinsic fluorescence. The fluorescent acrosomal matrix was observed inside the fertilized egg and remained structurally intact even after gastrulation started. This observation suggests that FAD is not released from the acrosomal matrix during the fertilization process or early development and supports an idea that FAD is involved in the formation of the acrosomal matrix. The intrinsic fluorescence of the A. remigis acrosome will be a useful marker for following spermatogenesis and fertilization.
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影响因子：
1.4
作者：
Campbell, V;Fairbairn, DJ
通讯作者：
Fairbairn, DJ
DOI：
10.1007/bf00267822
发表时间：
1993-12-01
期刊：
HISTOCHEMISTRY
影响因子：
--
作者：
OHTANI, H;WAKUI, H;MIURA, AB
通讯作者：
MIURA, AB
影响因子：
1.5
作者：
Pollister, AW
通讯作者：
Pollister, AW
DOI：
10.1073/pnas.0630280100
发表时间：
2003-03-18
影响因子：
11.1
作者：
Haralampus-Grynaviski, NM;Lamb, LE;Simon, JD
通讯作者：
Simon, JD
DOI：
10.1111/j.1365-2818.1995.tb03669.x
发表时间：
1995-11-01
期刊：
JOURNAL OF MICROSCOPY-OXFORD
影响因子：
--
作者：
OLDENBOURG, R;MEI, G
通讯作者：
MEI, G