Amine-functionalized carbon-fiber microelectrodes for enhanced ATP detection with fast-scan cyclic voltammetry.
Amine-functionalized carbon-fiber microelectrodes for enhanced ATP detection with fast-scan cyclic voltammetry.
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DOI：
10.1039/d1ay00089f
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发表时间：
2021-05-27
期刊：
影响因子：
--
通讯作者：
Ross AE
中科院分区：
文献类型：
--
作者：
Li Y;Weese ME;Cryan MT;Ross AE
在这里，我们提供的证据表明，用胺对碳纤维表面进行功能化处理显著提高了快速扫描循环伏安法(FSCV)直接检测三磷酸腺苷(ATP)的性能。三磷酸腺苷是一种重要的细胞外信号分子，在人体内起到神经递质的作用。多年来，已经发展了几种方法来监测和定量细胞和组织中的ATP信号；然而，其中许多方法在时间分辨率上受到限制，或者不能直接测量ATP。碳纤维微电极上的FSCV是一种广泛使用的实时测量神经递质的技术。已经开发了许多电极处理方法来研究多巴胺等阳离子化合物在碳表面的相互作用，但缺乏研究如何改善碳纤维表面的阴离子化合物，如三磷酸腺苷。在本工作中，碳纤维用乙二胺(N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide)与碳表面的羧酸基反应，然后再与乙二胺反应，得到氨基功能化的碳表面。总体而言，通过X射线光电子能谱分析，经过EDC-EDA修饰后，碳表面的ATP电流增加了5.2±2.5倍，胺的官能度增加了约9倍。这提供了富含胺的表面改善与表面上的ATP的相互作用的证据。本研究对碳表面的三磷酸腺苷相互作用进行了详细的分析，并最终提出了一种改进未来神经学三磷酸腺苷直接快速检测的方法。胺功能化碳纤维微电极显著改善了快速扫描循环伏安法(FSCV)直接检测三磷酸腺苷(ATP)的性能。
Here, we provide evidence that functionalizing the carbon-fiber surface with amines significantly improves direct electrochemical adenosine triphosphate (ATP) detection with fast-scan cyclic voltammetry (FSCV). ATP is an important extracellular signaling molecule throughout the body and can function as a neurotransmitter in the brain. Several methods have been developed over the years to monitor and quantitate ATP signaling in cells and tissues; however, many of them are limited in temporal resolution or are not capable of measuring ATP directly. FSCV at carbon-fiber microelectrodes is a widely used technique to measure neurotransmitters in real-time. Many electrode treatments have been developed to study the interaction of cationic compounds like dopamine at the carbon surface yet studies investigating how to improve anionic compounds, like ATP, at the carbon fiber surface are lacking. In this work, carbon-fibers were treated with N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide (EDC) which reacts with carboxylic acid groups on the carbon surface followed by reaction with ethylenediamine (EDA) to produce NH2-functionalized carbon surfaces. Overall, we see a 5.2 ± 2.5 -fold increase in ATP current with an approximately 9-fold increase in amine functionality, as analyzed by X-ray Photoelectron Spectroscopy, on the carbon surface after modification with EDC-EDA. This provides evidence that amine-rich surfaces improve interactions with ATP on the surface. This study provides a detailed analysis of ATP interaction at carbon surfaces and ultimately a method to improve direct and rapid neurological ATP detection in the future. Amine functionalized carbon-fiber microelectrodes significantly improve direct adenosine triphosphate (ATP) detection with fast-scan cyclic voltammetry (FSCV).
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影响因子：
5.6
作者：
Di Liberto V;Mudò G;Garozzo R;Frinchi M;Fernandez-Dueñas V;Di Iorio P;Ciccarelli R;Caciagli F;Condorelli DF;Ciruela F;Belluardo N
通讯作者：
Belluardo N
影响因子：
8.9
作者：
Lim, Gary N.;Ross, Ashley E.
通讯作者：
Ross, Ashley E.
影响因子：
4.2
作者：
Heien, MLAV;Phillips, PEM;Wightman, RM
通讯作者：
Wightman, RM
DOI：
10.1016/j.jchromb.2012.02.005
发表时间：
2012-03-15
影响因子：
3
作者：
Bhatt, Dhaval P.;Chen, Xuesong;Geiger, Jonathan D.;Rosenberger, Thad A.
通讯作者：
Rosenberger, Thad A.
影响因子：
3.5
作者：
Dale, Nicholas;Frenguelli, Bruno G.
通讯作者：
Frenguelli, Bruno G.