Early detection of burn induced heterotopic ossification using transcutaneous Raman spectroscopy.
Early detection of burn induced heterotopic ossification using transcutaneous Raman spectroscopy.
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DOI：
10.1016/j.bone.2013.01.002
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发表时间：
2013-05
期刊：
影响因子：
4.1
通讯作者：
Levi, Benjamin
中科院分区：
文献类型：
--
作者：
Peterson, Jonathan R.;Okagbare, Paul I.;De La Rosa, Sara;Cilwa, Katherine E.;Perosky, Joseph E.;Eboda, Oluwatobi N.;Donneys, Alexis;Su, Grace L.;Buchman, Steven R.;Cederna, Paul S.;Wang, Stewart C.;Kozloff, Kenneth M.;Morris, Michael D.;Levi, Benjamin
异位骨化（HO），或软组织骨的异常形成，发生在60%以上的严重烧伤和爆炸创伤。目前的诊断模式不足以在早期诊断和干预HO，迫切需要改进。拉曼光谱已经在以前的研究中用于报道骨发育过程中骨成分的变化，但尚未应用于烧伤诱导的HO。在这项研究中，我们验证了经皮体内拉曼光谱作为烧伤后小鼠HO早期诊断的方法。采用跟腱切开术模型研究HO的形成。肌腱切开后，将小鼠分为烧伤组和假手术组，分别将背部30%表面积暴露于60°水或30°水中18秒。在体内，在肌腱切断和未受伤的腿的早期时间点（5天、2周和3周）和晚期时间点（3个月）进行经皮拉曼光谱分析。然后将这些相同的样本解剖到骨头，并对切除的组织进行离体拉曼测量。在相应的时间点用显微CT和组织学检查证实骨形成。我们的拉曼探针允许非侵入性，经皮评估异位骨形成。Raman数据显示，与对照组相比，肌腱切断术的骨矿物质信号在损伤后5天显著增加，随着时间的推移差异越来越大，而Micro CT直到三周才显示骨异位。离体拉曼测量显示，与假手术组相比，烧伤组HO的量有显著差异，并且与先前存在的皮质骨相比，新生异位骨的光谱也有差异。当烧伤合并外伤性损伤时，会增加发生HO的可能性。在我们的体内小鼠模型中，拉曼光谱可以在损伤后5天检测到HO的形成。新骨的骨矿物质和基质组成的变化也在拉曼光谱中得到证实，这有助于早期识别HO，并为HO患者提供更及时的治疗决策。
Heterotopic ossification (HO), or the abnormal formation of bone in soft tissue, occurs in over 60% of major burn injuries and blast traumas. A significant need exists to improve the current diagnostic modalities for HO which are inadequate to diagnose and intervene on HO at early time-points. Raman spectroscopy has been used in previous studies to report on changes in bone composition during bone development but has not yet been applied to burn induced HO. In this study, we validate transcutaneous, in-vivo Raman spectroscopy as a methodology for early diagnosis of HO in mice following a burn injury. An Achilles tenotomy model was used to study HO formation. Following tenotomy, mice were divided into burn and sham groups with exposure of 30% surface area on the dorsum to 60° water or 30° water for 18 seconds respectively. In-vivo, transcutaneous Raman spectroscopy was performed at early time points (5 days, 2 and 3 weeks) and a late time point (3 months) on both the tenotomized and non-injured leg. These same samples were then dissected down to the bone and ex-vivo Raman measurements were performed on the excised tissue. Bone formation was verified with Micro CT and histology at corresponding time-points. Our Raman probe allowed non-invasive, transcutaneous evaluation of heterotopic bone formation. Raman data showed significantly increased bone mineral signaling in the tenotomy compared to control leg at 5 days post injury, with the difference increasing over time whereas Micro CT did not demonstrate heterotopic bone until three weeks. Ex-vivo Raman measurements showed significant differences in the amount of HO in the burn compared to sham groups and also showed differences in the spectra of new, ectopic bone compared to pre-existing cortical bone. Burn injury increases the likelihood of developing HO when combined with traumatic injury. In our in-vivo mouse model, Raman spectroscopy allowed for detection of HO formation as early as 5 days post injury. Changes in bone mineral and matrix composition of the new bone were also evidenced in the Raman spectra which could facilitate early identification of HO and allow more timely therapy decisions for HO patients.
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影响因子：
3.5
作者：
Schulmerich, Matthew V.;Cole, Jacqueline H.;Goldstein, Steven A.
通讯作者：
Goldstein, Steven A.
DOI：
10.1038/sc.1982.39
发表时间：
1982-01-01
期刊：
PARAPLEGIA
影响因子：
--
作者：
FREED, JH;HAHN, H;DILLON, T
通讯作者：
DILLON, T
影响因子：
4.8
作者：
Levi, Benjamin;Wan, Derrick C.;Longaker, Michael T.
通讯作者：
Longaker, Michael T.
影响因子：
3.8
作者：
Ipaktchi, Kyros;Mattar, Aladdein;Arbabi, Saman
通讯作者：
Arbabi, Saman
影响因子：
4.1
作者：
Meganck, Jeffrey A.;Kozloff, Kenneth M.;Thornton, Michael M.;Broski, Stephen M.;Goldstein, Steven A.
通讯作者：
Goldstein, Steven A.