Identification and characterization of epiphytic yeasts on apples in China

Identification and characterization of epiphytic yeasts on apples in China
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中国苹果附生酵母菌的鉴定与表征

DOI:
10.1039/c7ra08234g
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发表时间:
2017-09
期刊:
影响因子:
3.9
通讯作者:
Tianli Yue
Tianli Yue
中科院分区:
化学3区
文献类型:
--
作者:
Jianping Wei;Chen Niu;Bin Liu;Yahong Yuan;Tianli Yue

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目前,关于苹果上附生酵母的流行和多样性知之甚少。因此,在这项研究中,我们试图确定和表征酵母对苹果的潜在苹果酒酿造性能。从我国两大生态区的8个省(市、区)的苹果皮中分离到754株酵母菌,黄土高原和渤海湾地区。共鉴定出24属71种,其中Aureobasidium(A.)pullulans和Hanseniaspora(H.)葡萄囊是最主要的酵母种类。我们在相关的苹果样品上没有观察到任何酿酒酵母菌株。对应分析表明,陕西的汉逊酵母属、辽宁的红酵母属、山东的假丝酵母属、山西的短梗霉属和河北的毕赤酵母属与各省区有一定的联系。甘肃、河南、宁夏苹果中未发现优势属。虽然23个H.葡萄囊菌菌株进行了鉴定,有没有明确的联系,菌株之间的起源,基因型和表型的RAPD-PCR分析和一些定性评价的酵母生化活性。
Currently, little is known regarding the prevalence and diversity of epiphytic yeasts on apples. Therefore, in this study we attempted to identify and characterize yeasts on apples for their potential cider-making performance. A total of 754 yeast strains were isolated from the skins of apples collected from eight provinces in the two main ecological regions of China, i.e., the Loess Plateau and Bo Hai Gulf regions. More than 71 species belonging to 24 genera were identified and Aureobasidium (A.) pullulans and Hanseniaspora (H.) uvarum were the most predominant yeast species. We did not observe any Saccharomyces cerevisiae strains on the associated apple samples. Correspondence analysis revealed the following connection between yeast genera and the provinces: Hanseniaspora in Shaanxi, Rhodotorula in Liaoning, Candida in Shandong, Aureobasidium in Shanxi and Pichia in Hebei. No dominant genera were observed in apples from Gansu, Henan and Ningxia. Although genetic differences among the 23 H. uvarum strains were identified, there was no clear link between the isolates as to origin, genotype and phenotype following RAPD-PCR analysis and some qualitative evaluation of the yeast biochemical activities.
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