Effect of vitamin E on hepatic cell proliferation and apoptosis in mice deficient in the p50 subunit of NF-κB after treatment with phenobarbital.
Effect of vitamin E on hepatic cell proliferation and apoptosis in mice deficient in the p50 subunit of NF-κB after treatment with phenobarbital.
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DOI：
10.1016/j.fct.2011.06.059
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发表时间：
2011-10
影响因子：
4.3
通讯作者：
Glauert, Howard P.
中科院分区：
文献类型：
--
作者：
Li, Jun;Harp, Casey;Tharappel, Job C.;Spear, Brett T.;Glauert, Howard P.
苯巴比妥（PB）是一种有效的肝肿瘤促进剂，研究较多。核因子κB（NF-κB）是一种由活性氧激活的转录因子，参与细胞增殖和凋亡。我们以前发现PB激活NF-κB，膳食维生素E可有效降低PB诱导的NF-κB DNA结合。因此，我们假设膳食维生素E通过其对NF-κB的作用影响PB诱导的细胞增殖和凋亡的变化。对NF-κB1缺陷小鼠（p50−/−）和野生型B6129小鼠喂食含有10或250 ppm维生素E（α-生育酚乙酸酯）的纯化饲料28天。在那个时候，一半的野生型和一半的p50−/−小鼠被置于相同的饮食与0.05%的PB 10天。与野生型小鼠相比，p50−/−小鼠的细胞增殖和凋亡水平更高。PB可显著促进细胞增殖，但维生素E对肝细胞增殖无影响。PB组细胞凋亡无明显变化，对照组和高维生素E组细胞凋亡无明显差异。因此，维生素E似乎不会影响野生型或p50−/−小鼠的细胞生长参数。
Phenobarbital (PB) is an efficacious and well-studied hepatic tumor promoting agent. Nuclear factorκB (NF-κB) is a transcription factor activated by reactive oxygen and is involved in cell proliferation and apoptosis. We previously found that PB activates NF-κB and that dietary vitamin E is effective in decreasing PB-induced NF-κB DNA binding. We therefore hypothesized that dietary vitamin E influences PB-induced changes in cell proliferation and apoptosis through its action on NF-κB. NF-κB1 deficient mice (p50−/−) and wild-type B6129 mice were fed a purified diet containing 10 or 250 ppm vitamin E (α-tocopherol acetate) for 28 days. At that time, half of the wild-type and half of the p50−/− mice were placed on the same diet with 0.05% PB for 10 days. Compared to wild-type mice, the p50−/− mice had higher levels of cell proliferation and apoptosis. Cell proliferation was significantly increased by PB, but vitamin E did not affect hepatic cell proliferation. Apoptosis was not changed in mice fed PB, and there was no significant difference in apoptosis between control and high vitamin E treated mice. Thus, vitamin E does not appear to influence cell growth parameters in either wild-type or p50−/− mice.
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影响因子：
4.3
作者：
Glauert, Howard P.;Tharappel, Job C.;Spear, Brett T.
通讯作者：
Spear, Brett T.
影响因子：
4.7
作者：
PITOT, HC;GOLDSWORTHY, TL;CAMPBELL, HA
通讯作者：
CAMPBELL, HA
影响因子：
3.8
作者：
Tharappel, JC;Nalca, A;Spear, BT
通讯作者：
Spear, BT
DOI：
10.1006/bbrc.1996.1911
发表时间：
1996-12-24
影响因子：
3.1
作者：
Li, YX;Leung, LK;Glauert, HP
通讯作者：
Glauert, HP
影响因子：
2.9
作者：
Glauert, Howard P.;Calfee-Mason, Karen;Li, Yixin;Nilakantan, Vani;Twaroski, Michelle L.;Tharappel, Job;Spear, Brett T.
通讯作者：
Spear, Brett T.