Sugarcane mosaic virus orchestrates the lactate fermentation pathway to support its successful infection.
Sugarcane mosaic virus orchestrates the lactate fermentation pathway to support its successful infection.
复制标题
甘蔗花叶病毒协调乳酸发酵途径以支持其成功感染
DOI：
10.3389/fpls.2022.1099362
复制
发表时间：
2022
影响因子：
5.6
通讯作者：
Zhou, Tao
中科院分区：
文献类型：
--
作者：
Jiang, Tong;Du, Kaitong;Wang, Pei;Wang, Xinhai;Zang, Lianyi;Peng, Dezhi;Chen, Xi;Sun, Geng;Zhang, Hao;Fan, Zaifeng;Cao, Zhiyan;Zhou, Tao
关键词：
病毒通常通过改变宿主的新陈代谢来建立自己的感染。病毒如何利用植物新陈代谢来支持它们的成功感染仍然是一个悬而未决的问题。在这里，我们使用非靶向代谢组学来揭示，在强烈的甘蔗花叶病毒(SCMV)感染之前和之后，乳酸立即积累。诱导乳酸参与的厌氧糖酵解有利于SCMV的感染。SCMV感染可上调乳酸脱氢酶(LDH)的活性和转录水平，LDH是SCMV强势感染所必需的。此外，LDH通过与SCMV编码的6K2蛋白相互作用在病毒复制酶复合体(VRCs)中重新定位，6K2蛋白是诱导VRCs的关键蛋白。此外，乳酸还可通过抑制植物的防御反应促进SCMV的侵染。综上所述，我们揭示了一种病毒策略，即操纵宿主新陈代谢以支持复制间隔，同时也抑制感染过程中的防御反应。
Viruses often establish their own infection by altering host metabolism. How viruses co-opt plant metabolism to support their successful infection remains an open question. Here, we used untargeted metabolomics to reveal that lactate accumulates immediately before and after robust sugarcane mosaic virus (SCMV) infection. Induction of lactate-involved anaerobic glycolysis is beneficial to SCMV infection. The enzyme activity and transcriptional levels of lactate dehydrogenase (LDH) were up-regulated by SCMV infection, and LDH is essential for robust SCMV infection. Moreover, LDH relocates in viral replicase complexes (VRCs) by interacting with SCMV-encoded 6K2 protein, a key protein responsible for inducing VRCs. Additionally, lactate could promote SCMV infection by suppressing plant defense responses. Taken together, we have revealed a viral strategy to manipulate host metabolism to support replication compartment but also depress the defense response during the process of infection.
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影响因子：
5.4
作者：
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通讯作者：
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影响因子：
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影响因子：
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作者：
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通讯作者：
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