High-throughput detection of ethanol-producing cyanobacteria in a microdroplet platform.
High-throughput detection of ethanol-producing cyanobacteria in a microdroplet platform.
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DOI：
10.1098/rsif.2015.0216
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发表时间：
2015-05-06
期刊：
影响因子：
--
通讯作者：
Abell C
中科院分区：
文献类型：
--
作者：
Abalde-Cela S;Gould A;Liu X;Kazamia E;Smith AG;Abell C
微生物生产乙醇是一种重要的可再生能源。大多数过程涉及从植物原料中发酵糖，但人们对由光合作用生物直接生产乙醇越来越感兴趣。为了促进这一点，需要一种用于检测乙醇的高通量筛选技术。在这里，描述了一种在基于微滴的平台上定量检测乙醇的方法，该方法可用于筛选蓝藻菌株以鉴定那些具有最高乙醇产量水平的菌株。对原液和微滴中乙醇的酶促检测方法进行了优化。同时，还研究了工程菌在微滴中的包埋及其在微滴库中的生长动力学。结合模块化的微滴操作，包括蓝藻胶囊的液滴生成，液滴重新注入和微微注射，以及激光诱导荧光，被用来创建这个新的平台来筛选不同乙醇生产水平的蓝藻基因工程菌株。
Ethanol production by microorganisms is an important renewable energy source. Most processes involve fermentation of sugars from plant feedstock, but there is increasing interest in direct ethanol production by photosynthetic organisms. To facilitate this, a high-throughput screening technique for the detection of ethanol is required. Here, a method for the quantitative detection of ethanol in a microdroplet-based platform is described that can be used for screening cyanobacterial strains to identify those with the highest ethanol productivity levels. The detection of ethanol by enzymatic assay was optimized both in bulk and in microdroplets. In parallel, the encapsulation of engineered ethanol-producing cyanobacteria in microdroplets and their growth dynamics in microdroplet reservoirs were demonstrated. The combination of modular microdroplet operations including droplet generation for cyanobacteria encapsulation, droplet re-injection and pico-injection, and laser-induced fluorescence, were used to create this new platform to screen genetically engineered strains of cyanobacteria with different levels of ethanol production.
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影响因子：
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