Characterization of a Caenorhabditis elegans recA-like gene Ce-rdh-1 involved in meiotic recombination.
Characterization of a Caenorhabditis elegans recA-like gene Ce-rdh-1 involved in meiotic recombination.
复制标题
参与减数分裂重组的秀丽隐杆线虫recA样基因Ce-rdh-1的表征。
作者：
T. Takanami;Shusei Sato;Takeshi Ishihara;Isao Katsura;Hideyuki Takahashi;Atsushi Higashitani
在秀丽隐杆线虫基因组计划数据库中发现了一个recA样基因。该基因的推定产物命名为Ce-rdh-1（C. elegans RAD 51和DMC 1/LIM 15同源物1），由357个氨基酸残基组成。Ce-rdh-1的氨基酸序列与几种真核生物中的RAD 51和DMC 1/LIM 15基因的同源性为46-60%。RNAi（RNA-mediated interference）结果表明，Ce-rdh-1的阻遏作用阻断了F1代卵母细胞中6个二价体的染色体凝聚和交叉的解离。卵子发生没有进行到终变期。因此，所产生的所有卵（F2）在早期阶段死亡。这些结果表明，Ce-rdh-1参与减数分裂重组。
A recA-like gene was identified in the Caenorhabditis elegans genome project database. The putative product of the gene, termed Ce-rdh-1 (C. elegans RAD51 and DMC1/LIM15 homolog 1), consists of 357 amino acid residues. The predicted amino acid sequence of Ce-rdh-1 showed 46-60% identity to both RAD51 type and DMC1/LIM15 type genes in several eukaryote species. The results of RNAi (RNA-mediated interference) indicated that repression of Ce-rdh-1 blocked chromosome condensation of six bivalents and dissociation of chiasmata in oocytes of F1 progeny. Oogenesis did not proceed to the diakinesis stage. Accordingly, all the eggs produced (F2) died in early stages. These results suggest that Ce-rdh-1 participates in meiotic recombination.
DMC1 在酿酒酵母减数分裂途径中发挥作用，该途径很大程度上独立于 RAD51 途径。
影响因子：
3.3
作者：
Dresser,ME;Ewing,DJ;Conrad,MN;Dominguez,AM;Barstead,R;Jiang,H;Kodadek,T
通讯作者：
Kodadek,T
影响早期秀丽隐杆线虫胚胎减数分裂和有丝分裂的突变。
影响因子：
3.3
作者：
Mains,PE;Kemphues,KJ;Sprunger,SA;Sulston,IA;Wood,WB
通讯作者：
Wood,WB
影响因子：
16
作者：
Pittman, DL;Cobb, J;Schimenti, JC
通讯作者：
Schimenti, JC