Immunologic Profiling of the Atlantic Salmon Gill by Single Nuclei Transcriptomics.
Immunologic Profiling of the Atlantic Salmon Gill by Single Nuclei Transcriptomics.
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单核转录组学对大西洋鲑鱼g的免疫学分析。
DOI：
10.3389/fimmu.2021.669889
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发表时间：
2021
影响因子：
7.3
通讯作者：
Hazlerigg DG
中科院分区：
文献类型：
--
作者：
West AC;Mizoro Y;Wood SH;Ince LM;Iversen M;Jørgensen EH;Nome T;Sandve SR;Martin SAM;Loudon ASI;Hazlerigg DG
溯河产卵的鲑鱼开始适应其纳塔尔河流的淡水环境的生活之前，发育过渡，称为银化，将它们转变为海洋适应性鱼类。在野生环境中，银化是一个光周期调节的过程，涉及鳃功能的彻底重塑，以科普海水（SW）迁移的深刻渗透和免疫挑战。虽然先前的工作已经强调了专门的“富含钙离子”细胞（MRC）和辅助细胞（AC）在传递这种表型中的作用，但最近的RNA分析实验表明，重塑比以前认识到的要广泛得多。在这里，我们使用单核RNAseq来表征银化和SW转移期间大西洋鲑鱼鳃中细胞学变化的程度。我们确定了20个不同的细胞簇，包括已知的，但也新的鳃细胞类型。这些数据使我们能够隔离集群特定的，银化相关的基因表达的变化，并描述如何通过银化的鳃细胞组成的变化。正如预期的那样，我们注意到海水中富含蛋白质的细胞比例增加，然而，我们也发现了几种免疫相关细胞类型以前未知的减少。总的来说，我们的研究结果提供了新的细节，在鳃细胞的复杂性，并表明银化触发意想不到的免疫重编程。
Anadromous salmonids begin life adapted to the freshwater environments of their natal streams before a developmental transition, known as smoltification, transforms them into marine-adapted fish. In the wild, smoltification is a photoperiod-regulated process, involving radical remodeling of gill function to cope with the profound osmotic and immunological challenges of seawater (SW) migration. While prior work has highlighted the role of specialized “mitochondrion-rich” cells (MRCs) and accessory cells (ACs) in delivering this phenotype, recent RNA profiling experiments suggest that remodeling is far more extensive than previously appreciated. Here, we use single-nuclei RNAseq to characterize the extent of cytological changes in the gill of Atlantic salmon during smoltification and SW transfer. We identify 20 distinct cell clusters, including known, but also novel gill cell types. These data allow us to isolate cluster-specific, smoltification-associated changes in gene expression and to describe how the cellular make-up of the gill changes through smoltification. As expected, we noted an increase in the proportion of seawater mitochondrion-rich cells, however, we also identify previously unknown reduction of several immune-related cell types. Overall, our results provide fresh detail of the cellular complexity in the gill and suggest that smoltification triggers unexpected immune reprogramming.
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影响因子：
64.5
作者：
Geijtenbeek, TBH;Torensma, R;Figdor, CG
通讯作者：
Figdor, CG
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
2.8
作者：
Braun, Marvin H.;Steele, Shelby L.;Perry, Steve F.
通讯作者：
Perry, Steve F.
影响因子：
4.4
作者：
Beertsen, Wouter;Willenborg, Marion;Saftig, Paul
通讯作者：
Saftig, Paul
影响因子：
2.7
作者：
AakuSaraste, E;Hellwig, A;Huttner, WB
通讯作者：
Huttner, WB