ITRAQ-based quantitative proteomic analysis of japonica rice seedling during cold stress.
ITRAQ-based quantitative proteomic analysis of japonica rice seedling during cold stress.
复制标题
基于iTRAQ的水稻幼苗低温胁迫蛋白质组定量分析。
DOI：
10.1270/jsbbs.21081
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发表时间：
2022-04
期刊：
影响因子：
2.4
通讯作者：
中科院分区：
文献类型：
--
作者：
低温是影响水稻生长和产量的重要环境因子之一。为了更好地了解粳稻对冷胁迫的反应，采用基于iTRAQ标记的定量蛋白质组学方法检测粳稻蛋白质水平的变化。以2周龄的耐冷水稻品种空育131幼苗为材料，在8 ℃下处理24、48和72 h，提取组织总蛋白，进行蛋白质组学定量分析。共检测到5082个蛋白质进行定量分析，其中289个蛋白质表达显著上调，其中169个蛋白质表达上调，125个蛋白质表达下调。功能分析表明，这些蛋白质主要参与光合作用、代谢途径、次生代谢产物的合成和碳代谢。蛋白质印迹分析表明，蛋白质调控与iTRAQ数据一致。对25个调控蛋白的相应基因进行了定量真实的时间PCR分析，结果表明mRNA水平与相应蛋白水平并不总是平行的。本研究的重要意义在于从蛋白质组学角度对水稻冷胁迫反应提供了新的认识，并为水稻耐冷育种提供了候选基因。
Low temperature is one of the important environmental factors that affect rice growth and yield. To better understand the japonica rice responses to cold stress, isobaric tags for a relative and absolute quantification (iTRAQ) labeling-based quantitative proteomics approach was used to detected changes in protein levels. Two-week-old seedlings of the cold tolerant rice variety Kongyu131 were treated at 8°C for 24, 48 and 72 h, then the total proteins were extracted from tissues and used for quantitative proteomics analysis. A total of 5082 proteins were detected for quantitative analysis, of which 289 proteins were significantly regulated, consisting of 169 uniquely up-regulated proteins and 125 uniquely down-regulated proteins in cold stress groups relative to the control group. Functional analysis revealed that most of the regulated proteins are involved in photosynthesis, metabolic pathway, biosynthesis of secondary metabolites and carbon metabolism. Western blot analysis showed that protein regulation was consistent with the iTRAQ data. The corresponding genes of 25 regulated proteins were used for quantitative real time PCR analysis, and the results showed that the mRNA level was not always parallel to the corresponding protein level. The importance of our study is that it provides new insights into cold stress responses in rice with respect to proteomics and provides candidate genes for cold-tolerance rice breeding.
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影响因子：
3.4
作者：
Hashimoto, Makoto;Komatsu, Setsuko
通讯作者：
Komatsu, Setsuko
影响因子：
4.8
作者：
Karlson, D;Nakaminami, K;Imai, R
通讯作者：
Imai, R
影响因子：
3
作者：
Ji, Li;Zhou, Ping;Qiu, Yongfu
通讯作者：
Qiu, Yongfu
野生稻 (Oryza rufipogon Griff.) QTL CTS-12 的比较蛋白质组学分析在水稻 (Oryza sativa L.) 应对严重低温胁迫的冷驯化和去驯化调节中的作用
影响因子：
5.3
作者：
Cen W;Liu J;Lu S;Jia P;Yu K;Han Y;Li R;Luo J
通讯作者：
Luo J
DOI：
10.1111/nph.14977
发表时间：
2018-04
期刊：
The New phytologist
影响因子：
--
作者：
Chen L;Zhao Y;Xu S;Zhang Z;Xu Y;Zhang J;Chong K
通讯作者：
Chong K