Comparative proteomic analysis of QTL CTS-12 derived from wild rice (Oryza rufipogon Griff.), in the regulation of cold acclimation and de-acclimation of rice (Oryza sativa L.) in response to severe chilling stress.
Comparative proteomic analysis of QTL CTS-12 derived from wild rice (Oryza rufipogon Griff.), in the regulation of cold acclimation and de-acclimation of rice (Oryza sativa L.) in response to severe chilling stress.
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野生稻 (Oryza rufipogon Griff.) QTL CTS-12 的比较蛋白质组学分析在水稻 (Oryza sativa L.) 应对严重低温胁迫的冷驯化和去驯化调节中的作用
DOI：
10.1186/s12870-018-1381-7
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发表时间：
2018-08-10
影响因子：
5.3
通讯作者：
Luo J
中科院分区：
文献类型：
--
作者：
Cen W;Liu J;Lu S;Jia P;Yu K;Han Y;Li R;Luo J
研究背景水稻（Oryza sativa L.）是一种易受低温胁迫的喜温作物。普通野生稻（Oryza rufipogonGriff.）在广西（中国）具有耐冷胁迫的能力。为了更好地了解野生稻耐冷性的分子机制，利用基于iTRAQ的蛋白质组学分析方法，对来自普通野生稻的一个主要耐冷性QTL CTS-12进行了检测，介导的低温和恢复诱导的差异表达蛋白质（DEPs），耐冷水稻品系DC 90和低温-恢复诱导的差异表达蛋白质（DEPs），结果9311和DC 90在整个低温和恢复期分别检测到206个和155个DEP。这些DEP在9311中聚成6个功能群，在DC 90中聚成4个功能群。大多数是丰富的“核糖体的结构组成”，“蛋白质-生色团连接”，和“光合作用和光捕获”类别。短时间序列表达数据挖掘（STEM）分析表明，9311和DC 90叶绿体光合和核糖体蛋白的动态变化具有明显差异。结论CTS-12可能介导了低温（冷驯化）和恢复（去驯化）条件下DC 90叶绿体光合和核糖体蛋白的动态变化，从而提高了该水稻品系的耐冷性。鉴定的DEP和CTS-12在蛋白质组水平介导DC 90的动态响应的参与阐明并加深了对野生稻耐冷性机制的理解。
BackgroundRice (Oryza sativaL.) is a thermophilic crop vulnerable to chilling stress. However, common wild rice (Oryza rufipogonGriff.) in Guangxi (China) has the ability to tolerate chilling stress. To better understand the molecular mechanisms underlying chilling tolerance in wild rice, iTRAQ-based proteomic analysis was performed to examineCTS-12, a major chilling tolerance QTL derived from common wild rice, mediated chilling and recovery-induced differentially expressed proteins (DEPs) between the chilling-tolerant rice line DC90 and the chilling-sensitive 9311.ResultsComparative analysis identified 206 and 155 DEPs in 9311 and DC90, respectively, in response to the whole period of chilling and recovery. These DEPs were clustered into 6 functional groups in 9311 and 4 in DC90. The majority were enriched in the ‘structural constituent of ribosome’, ‘protein-chromophore linkage’, and ‘photosynthesis and light harvesting’ categories. Short Time-series Expression Miner (STEM) analysis revealed distinct dynamic responses of both chloroplast photosynthetic and ribosomal proteins between 9311 and DC90.ConclusionCTS-12might mediate the dynamic response of chloroplast photosynthetic and ribosomal proteins in DC90 under chilling (cold acclimation) and recovery (de-acclimation) and thereby enhancing the chilling stress tolerance of this rice line. The identified DEPs and the involvement ofCTS-12in mediating the dynamic response of DC90 at the proteomic level illuminate and deepen the understanding of the mechanisms that underlie chilling stress tolerance in wild rice.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
64.5
作者：
Ma, Yun;Dai, Xiaoyan;Chong, Kang
通讯作者：
Chong, Kang
影响因子：
5.6
作者：
Bai, Bin;Wu, Jun;Deng, Qi-Yun
通讯作者：
Deng, Qi-Yun
影响因子：
3
作者：
Ji, Li;Zhou, Ping;Qiu, Yongfu
通讯作者：
Qiu, Yongfu
影响因子：
5.6
作者：
Mu Q;Zhang W;Zhang Y;Yan H;Liu K;Matsui T;Tian X;Yang P
通讯作者：
Yang P