Effective photosensitization and selectivity in vivo of Candida Albicans by meso-tetra (N-methyl-4-pyridyl) porphine tetra tosylate.
Effective photosensitization and selectivity in vivo of Candida Albicans by meso-tetra (N-methyl-4-pyridyl) porphine tetra tosylate.
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DOI：
10.1002/lsm.21049
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发表时间：
2011-04
影响因子：
2.4
通讯作者：
Foster, Thomas H.
中科院分区：
文献类型：
--
作者：
Mitra, Soumya;Haidaris, Constantine G.;Snell, Sara B.;Giesselman, Benjamin R.;Hupcher, Steven M.;Foster, Thomas H.
真菌白色念珠菌通常会导致免疫受损患者的粘膜和皮肤感染。研究了光敏剂Meso-四(N-甲基-4-吡啶)卟啉四对甲苯磺酸盐(TMP-1363)在体外对白色念珠菌感染的光动力治疗(PDT)的效果及其在动物模型中的选择性。采用集落形成单位(CFU)法比较TMP-1363与亚甲基蓝(MB)对体外白念珠菌PDT的影响。将白色念珠菌酵母接种于耳廓真皮内，建立小鼠体内感染模型。感染后第2天，外用0.3mgmLTMP-1TMP-1363mgmLTMP-1TMP-1363TMP-−。照射后30min，照射剂量为90J cm−2，照射剂量为50 mW cm−2。照射后2 h处死大鼠，取耳组织匀浆，用菌落形成单位测定其生物负荷。在活体大视野和共聚焦荧光成像评估了光敏剂的定位与白色念珠菌的关系。与MB相比，TMP-1363的光敏化导致体外对白色念珠菌的杀伤率增加了3个对数以上。体内荧光成像显示TMP-1363对白色念珠菌有高度的选择性标记。与未经治疗的对照组相比，使用TMP-1363的PDT感染导致每耳CFU显著减少。接受PDT治疗的受感染耳朵随着时间的推移显示完全愈合，耳廓没有明显的损伤。我们的体外和体内研究结果支持TMP-1363介导的光动力疗法是一种可行的治疗念珠菌病的方法。
The fungus Candida albicans commonly causes mucosal and cutaneous infections in patients with impaired immunity. We investigated the effectiveness of the photosensitizer meso-tetra (N-methyl-4-pyridyl) porphine tetra tosylate (TMP-1363) in the photodynamic treatment (PDT) of C. albicans infection in vitro and its selectivity in an animal model. The efficacy of TMP-1363 in PDT of C. albicans in vitro was compared to that of methylene blue (MB) using a colony forming unit (CFU) assay. In vivo infection in the mouse was established by inoculation of C. albicans yeast in the intradermal space of the ear pinna. Two days post-infection, 0.3 mg mL−1 TMP-1363 was administered topically. Thirty minutes after TMP-1363 application, the ears were irradiated at 514 nm using a fluence of 90 J cm−2 delivered at an irradiance of 50 mW cm−2. The ears were excised 2 hours post-irradiation, homogenized and the organism burden was determined by a CFU assay. In vivo wide field and confocal fluorescence imaging assessed the localization of the photosensitizer in relationship to C. albicans. Photosensitization with TMP-1363 resulted in a greater than three-log increase in killing of C. albicans in vitro compared to MB. In vivo fluorescence imaging demonstrated a high degree of selective labeling of C. albicans by TMP-1363. PDT of infection using TMP-1363 resulted in a significant reduction in CFU/ear relative to untreated controls. Infected ears subjected to PDT displayed complete healing over time with no observable damage to the pinna. Our in vitro and in vivo findings support TMP-1363-mediated PDT as a viable therapeutic approach for the photodynamic treatment of candidiasis.
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影响因子：
2.2
作者：
Baldwin, T.;Sakthianandeswaren, A.;Handman, E.
通讯作者：
Handman, E.
影响因子：
4.9
作者：
Belazi, M;Velegraki, A;Karamitsos, D
通讯作者：
Karamitsos, D
影响因子：
3.3
作者：
Chabrier-Rosello, Yeissa;Foster, Thomas H.;Haidaris, Constantine G.
通讯作者：
Haidaris, Constantine G.
DOI：
10.1016/j.tripleo.2009.10.006
发表时间：
2010-03-01
期刊：
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
影响因子：
--
作者：
de Oliveira Mima, Ewerton Garcia;Pavarina, Ana Claudia;Bagnato, Vanderlei Salvador
通讯作者：
Bagnato, Vanderlei Salvador
DOI：
10.1111/j.1365-2818.1993.tb03313.x
发表时间：
1993-03-01
期刊：
JOURNAL OF MICROSCOPY-OXFORD
影响因子：
--
作者：
MANDERS, EMM;VERBEEK, FJ;ATEN, JA
通讯作者：
ATEN, JA