Defining essential stem cell characteristics in adipose-derived stromal cells extracted from distinct anatomical sites.
Defining essential stem cell characteristics in adipose-derived stromal cells extracted from distinct anatomical sites.
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DOI：
10.1007/s00441-012-1423-7
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发表时间：
2012-08
影响因子：
3.6
通讯作者：
Holt, Shawn E.
中科院分区：
文献类型：
--
作者：
Sachs, Patrick C.;Francis, Michael P.;Zhao, Min;Brumelle, Jenni;Rao, Raj R.;Elmore, Lynne W.;Holt, Shawn E.
脂肪来源的基质细胞(ASCs)的发现为患者特异性细胞替代疗法的发展创造了许多机会。我们已经从不同的解剖部位(腹部、手臂/腿、乳房、臀部)分离出多种ASCs细胞株，表明ASCs在全身广泛分布。不幸的是，文献中普遍缺乏关于它们的“干细胞”特征的一致意见。我们发现ASCs的端粒酶活性及其催化亚基的表达均低于检测水平，与年龄和培养条件无关。ASCs也会经历端粒磨损和最终衰老，同时保持稳定的核型，不会发生自发的肿瘤相关异常。使用一组已被推广用于识别ASCs的细胞表面标记，我们发现它们无法区分ASCs和正常成纤维细胞，因为两者都呈CD29、CD73和CD105阳性，而CD14、CD31和CD45呈阴性。所有ASC分离株都是多能的，能够分化为骨细胞、软骨细胞和脂肪细胞，而成纤维细胞没有分化潜力。与成纤维细胞和骨髓间充质干细胞(BM-MSCs)相比，我们的ASC株还表现出与多能细胞OCT-4、SOX2和NANOG相关的基因表达增加，尽管这些表达水平低于诱导的多能干细胞(IPS)。综上所述，我们的数据表明，尽管ASCs的细胞表面轮廓并不能将它们与正常的成纤维细胞区分开来，但其分化能力和与多能性密切相关的基因的表达清楚地将ASCs定义为多能干细胞，无论组织分离位置如何。
The discovery of adipose-derived stromal cells (ASCs) has created many opportunities for the development of patient-specific cell-based replacement therapies. We have isolated multiple cell strains of ASCs from various anatomical sites (abdomen, arms/legs, breast, buttocks), indicating wide-spread distribution of ASCs throughout the body. Unfortunately, there exists a general lack of agreement in the literature as to their “stem cell” characteristics. We find that telomerase activity and expression of its catalytic subunit in ASCs are both below the levels of detection, independent of age and culturing conditions. ASCs also undergo telomere attrition and eventually senesce, while maintaining a stable karyotype without the development of spontaneous tumor-associated abnormalities. Using a set of cell surface markers that have been promoted to identify ASCs, we find that they failed to distinguish ASCs from normal fibroblasts, as both are positive for CD29, CD73, and CD105 and negative for CD14, CD31, and CD45. All of the ASC isolates are multipotent, capable of differentiating into osteocytes, chondrocytes, and adipocytes, while fibroblasts show no differentiation potential. Our ASC strains also show elevated expression of genes associated with pluripotent cells, Oct-4, SOX2, and NANOG when compared to fibroblasts and bone marrow-derived mesenchymal stem cells (BM-MSCs), although the levels were lower than induced pluripotent stem cells (iPS). Together, our data suggest that while the cell surface profile of ASCs does not distinguish them from normal fibroblasts, their differentiation capacity and the expression of genes closely linked to pluripotency clearly define ASCs as multipotent stem cells, regardless of tissue isolation location.
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DOI：
10.1016/j.bbrc.2004.11.059
发表时间：
2005-01-21
影响因子：
3.1
作者：
Abdallah, BM;Haack-Sorensen, M;Kassem, M
通讯作者：
Kassem, M
影响因子：
7.4
作者：
Astori, Giuseppe;Vignati, Francesca;Moccetti, Tiziano
通讯作者：
Moccetti, Tiziano
影响因子：
3.2
作者：
Rebelatto, C. K.;Aguiar, A. M.;Correa, A.
通讯作者：
Correa, A.
影响因子：
5.2
作者：
Kern, Susanne;Eichler, Hermann;Bieback, Karen
通讯作者：
Bieback, Karen
影响因子：
2.6
作者：
Wagner, W;Wein, F;Ho, AD
通讯作者：
Ho, AD