Engine out of the chassis: cell-free protein synthesis and its uses.
Engine out of the chassis: cell-free protein synthesis and its uses.
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DOI：
10.1016/j.febslet.2013.10.016
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发表时间：
2014-01-21
期刊：
影响因子：
3.5
通讯作者：
Cooperman BS
中科院分区：
文献类型：
--
作者：
Rosenblum G;Cooperman BS
The translation machinery is the engine of life. Extracting the cytoplasmic milieu from a cell affords a lysate capable of producing proteins in concentrations reaching tens of micromolar. Such lysates, derivable from a variety of cells, allow the facile addition and subtraction of components that are directly or indirectly related to the translation machinery and/or the over-expressed protein. The flexible nature of such cell-free expression systems, when coupled with high throughput monitoring, can be especially suitable for protein engineering studies, allowing one to bypass multiple steps typically required using conventional in vivo protein expression.
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