A method for the selective depletion of microglia in the dorsal hippocampus in the juvenile rat brain.
A method for the selective depletion of microglia in the dorsal hippocampus in the juvenile rat brain.
复制标题
一种选择性去除幼年大鼠脑背海马小胶质细胞的方法。
DOI：
10.1016/j.jneumeth.2022.109567
复制
发表时间：
2022-05-15
影响因子：
3
通讯作者：
Schwarz, Jaclyn M.
中科院分区：
文献类型：
--
作者：
Hall, Mary Beth;Habash, Nicola M.;Haas, Nicole A.;Schwarz, Jaclyn M.
关键词：
为了了解小胶质细胞在大脑功能和发育中的作用，出现了耗尽整个大脑的小胶质细胞的方法。脂质体包裹的氯屈膦酸盐(LEC)可以按脑区和时间特定的方式注入大脑，以耗尽小胶质细胞。这项研究验证了在幼年发育的特定时期耗尽大鼠背海马(DHP)小胶质细胞的方法学。在出生后16天或19天进行立体定向手术，向DHP内注入LEC。不同年龄的大鼠脑组织被采集，以确定输注的特异性和耗竭的持续时间。P19用27G注射器将LEC注入DHP，从P24-P30开始，DHP的CA1、齿状回和CA3亚区的小胶质细胞被耗尽。在输液部位上方的顶叶皮质也有耗竭的证据。P16用32G注射器输注LEC仅在P21-P40的DHP亚区CA1和DG中耗尽小胶质细胞。以前的方法是将LEC注入成年大鼠的海马区或新生大鼠的脑室。这项研究首次发表了在幼年大鼠发育过程中以特定脑区方式消耗小胶质细胞的方法学。在幼年期输注LEC的时间可以调整，以在特定的出生后一天达到最大的小胶质细胞耗竭。27G针在输液过程中会导致LEC回流，但也允许LEC到达DHP的所有分区。使用32G针输液可防止输液过程中的返流，但会导致DHP内LEC的局部扩散。
To understand the role of microglia in brain function and development, methods have emerged to deplete microglia throughout the brain. Liposome-encapsulated clodronate (LEC) can be infused into the brain to deplete microglia in a brain-region and time-specific manner. This study validates methodology to deplete microglia in the rat dorsal hippocampus (dHP) during a specific period of juvenile development. Stereotaxic surgery was performed to infuse LEC at postnatal day (P) 16 or 19 into dHP. Rat brains were harvested at various ages to determine specificity of infusion and duration of depletion. P19 infusion of LEC into dHP with a 27G syringe depleted microglia in dHP subregions CA1, dentate gyrus (DG), and CA3 from P24-P30. There was also evidence of depletion in parietal cortex above the infusion site. P16 infusion of LEC with a 32G syringe depleted microglia only in dHP subregions CA1 and DG from P21-P40. Previous methods have infused LEC intra-hippocampally in adult rats or intra-cerebroventricularly in neonatal rats. This study is the first to publish methodology to deplete microglia in a brain-region specific manner during juvenile rat development. The timing of LEC infusion during the juvenile period can be adjusted to achieve maximal microglia depletion by a specific postnatal day. A 27G needle results in LEC backflow during the infusion, but also allows LEC to reach all subregions of dHP. Infusion with a 32G needle prevents backflow during infusion, but results in a more local spread of LEC within dHP.
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DOI：
10.1007/s00109-017-1573-x
发表时间：
2017-11
期刊：
Journal of molecular medicine (Berlin, Germany)
影响因子：
--
作者：
Faraco G;Park L;Anrather J;Iadecola C
通讯作者：
Iadecola C
DOI：
10.1523/jneurosci.2102-11.2011
发表时间：
2011-09-07
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Faustino JV;Wang X;Johnson CE;Klibanov A;Derugin N;Wendland MF;Vexler ZS
通讯作者：
Vexler ZS
影响因子：
3.7
作者：
Casanova F;Carney PR;Sarntinoranont M
通讯作者：
Sarntinoranont M
影响因子：
3.6
作者：
BIEWENGA, J;VANDERENDE, MB;VANROOIJEN, N
通讯作者：
VANROOIJEN, N
影响因子：
2.7
作者：
Schiffino, Felipe L.;Murawski, Nathen J.;Rosen, Jeffrey B.;Stanton, Mark E.
通讯作者：
Stanton, Mark E.