Modeling Neisseria meningitidis metabolism: from genome to metabolic fluxes.
Modeling Neisseria meningitidis metabolism: from genome to metabolic fluxes.
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DOI：
10.1186/gb-2007-8-7-r136
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发表时间：
2007
期刊：
影响因子：
12.3
通讯作者：
Martens DE
中科院分区：
文献类型：
--
作者：
Baart GJ;Zomer B;de Haan A;van der Pol LA;Beuvery EC;Tramper J;Martens DE
建立了脑膜炎奈瑟氏菌初级代谢的基因组尺度通量模型。使用该模型设计了脑膜炎病毒，并在分批培养和恒化器培养中成功地进行了测试。脑膜炎奈瑟菌是一种人类病原体，可以感染人类宿主的不同部位。N.脑膜炎是造成死亡和残疾的原因，特别是在幼儿中。总的来说，最近关于N.脑膜炎集中于潜在的抗原及其功能、免疫原性和致病机制。在过去的25年里，对奈瑟氏菌初级代谢的研究很少。利用N.结合文献中的生化和生理信息，我们构建了N.脑膜炎在恒化器培养物中使用通量平衡分析来检查来自基因组尺度代谢网络的简化代谢网络的有效性。从计算机实验中获得了几个有用的预测，包括底物偏好。N.脑膜炎病毒的设计和测试成功地在分批和恒化器培养。验证的代谢模型描述了氮素的初级代谢。恒化器中处于稳定状态的脑膜炎。基因组尺度模型是有价值的，因为它提供了一个框架，研究N。它可以作为一个整体或其某些方面的脑膜炎代谢，也可以用于疫苗工艺开发（例如，生长培养基的设计）。主要代谢途径（即戊糖磷酸途径和Entner-Douderoff途径）的通量分布表明，丙酮酸的大部分（69%）是通过ED切割合成的，这一发现与文献非常一致。
A genome-scale flux model for primary metabolism of Neisseria meningitidis was constructed; a minimal medium for growth of N. meningitidis was designed using the model and tested successfully in batch and chemostat cultures. Neisseria meningitidis is a human pathogen that can infect diverse sites within the human host. The major diseases caused by N. meningitidis are responsible for death and disability, especially in young infants. In general, most of the recent work on N. meningitidis focuses on potential antigens and their functions, immunogenicity, and pathogenicity mechanisms. Very little work has been carried out on Neisseria primary metabolism over the past 25 years. Using the genomic database of N. meningitidis serogroup B together with biochemical and physiological information in the literature we constructed a genome-scale flux model for the primary metabolism of N. meningitidis. The validity of a simplified metabolic network derived from the genome-scale metabolic network was checked using flux-balance analysis in chemostat cultures. Several useful predictions were obtained from in silico experiments, including substrate preference. A minimal medium for growth of N. meningitidis was designed and tested succesfully in batch and chemostat cultures. The verified metabolic model describes the primary metabolism of N. meningitidis in a chemostat in steady state. The genome-scale model is valuable because it offers a framework to study N. meningitidis metabolism as a whole, or certain aspects of it, and it can also be used for the purpose of vaccine process development (for example, the design of growth media). The flux distribution of the main metabolic pathways (that is, the pentose phosphate pathway and the Entner-Douderoff pathway) indicates that the major part of pyruvate (69%) is synthesized through the ED-cleavage, a finding that is in good agreement with literature.
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影响因子：
3.1
作者：
Bos, MP;Tommassen, J
通讯作者：
Tommassen, J
影响因子：
3.1
作者：
Dunn, KLR;Farrant, JL;Kroll, JS
通讯作者：
Kroll, JS
影响因子：
--
作者：
BENTHIN, S;NIELSEN, J;VILLADSEN, J
通讯作者：
VILLADSEN, J
影响因子：
36.8
作者：
CHEN, CY;GENCO, CA;MORSE, SA
通讯作者：
MORSE, SA
影响因子：
3.2
作者：
Erwin, AL;Gotschlich, EC
通讯作者：
Gotschlich, EC