Dean-flow-coupled elasto-inertial particle and cell focusing in symmetric serpentine microchannels
Dean-flow-coupled elasto-inertial particle and cell focusing in symmetric serpentine microchannels
复制标题
对称蛇形微通道中的迪恩流耦合弹惯性粒子和细胞聚焦
DOI：
10.1007/s10404-019-2204-3
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发表时间：
2019-02
影响因子：
2.8
通讯作者：
Li Weihua
中科院分区：
文献类型：
--
作者：
Yuan Dan;Sluyter Ronald;Zhao Qianbin;Tang Shiyang;Yan Sheng;Yun Guolin;Li Ming;Zhang Jun;Li Weihua
本文研究了对称蛇形微通道中Dean流耦合弹惯性效应下的粒子聚焦问题。在样品溶液中加入少量聚合物以调节流体弹性，使颗粒在弹性、惯性和Dean流力的综合作用下横向迁移并在对称蛇形通道的中心线上达到平衡。首先，研究了蛇形流道内粘弹性流体中流速对颗粒聚焦的影响。然后，与蛇形流道中牛顿流体和直线流道中粘弹性流体中的粒子聚焦进行了比较。弹性效应和蛇形流道结构可以加速颗粒的聚焦，减小流道长度。这种聚焦技术有可能成为流式细胞术中用于细胞计数、分类和分析的预排序单元。此外，还研究了Jurkat小室在蛇形流道中粘弹性流体中的聚焦行为。最后，在含有溶解聚合物的培养液中和通过蛇形通道处理后的细胞活力进行了测试。这种粘弹性流体中的聚合物对细胞活力的影响可以忽略不计。
This work investigates particle focusing under Dean-flow-coupled elasto-inertial effects in symmetric serpentine microchannels. A small amount of polymers were added to the sample solution to tune the fluid elasticity, and allow particles to migrate laterally and reach their equilibriums at the centerline of a symmetric serpentine channel under the synthesis effect of elastic, inertial and Dean-flow forces. First, the effects of the flow rates on particle focusing in viscoelastic fluid in serpentine channels were investigated. Then, comparisons with particle focusing in the Newtonian fluid in the serpentine channel and in the viscoelastic fluid in the straight channel were conducted. The elastic effect and the serpentine channel structure could accelerate the particle focusing as well as reduce the channel length. This focusing technique has the potential as a pre-ordering unit in flow cytometry for cell counting, sorting, and analysis. Moreover, focusing behaviour of Jurkat cells in the viscoelastic fluid in this serpentine channel was studied. Finally, the cell viability in the culture medium containing a dissolved polymer and after processing through the serpentine channel was tested. The polymer within this viscoelastic fluid has a negligible effect on cell viability.
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影响因子：
2.4
作者：
J. Heyman
通讯作者：
J. Heyman
影响因子：
6.1
作者：
D'Avino, Gaetano;Romeo, Giovanni;Maffettone, Pier Luca
通讯作者：
Maffettone, Pier Luca
粘弹性溶液中各种纳米粒子的无鞘聚焦和分离，最小化剪切稀化
影响因子：
7.4
作者：
Liu, Chao;Ding, Baoquan;Su, Jiashu
通讯作者：
Su, Jiashu
影响因子：
64.8
作者：
MacDonald, MP;Spalding, GC;Dholakia, K
通讯作者：
Dholakia, K
影响因子：
--
作者：
R. Vaidyanathan;Trifanny Yeo;C. Lim
通讯作者：
R. Vaidyanathan;Trifanny Yeo;C. Lim