Dietary restriction attenuates the accelerated aging phenotype of Sod1(-/-) mice.
Dietary restriction attenuates the accelerated aging phenotype of Sod1(-/-) mice.
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DOI：
10.1016/j.freeradbiomed.2013.02.026
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发表时间：
2013-07
影响因子：
7.4
通讯作者：
Van Remmen, Holly
中科院分区：
文献类型：
--
作者：
Zhang, Yiqiang;Ikeno, Yuji;Bokov, Alex;Gelfond, Jon;Jaramillo, Carlos;Zhang, Hong-Mei;Liu, Yuhong;Qi, Wenbo;Hubbard, Gene;Richardson, Arlan;Van Remmen, Holly
饮食限制是一种强大的衰老干预措施，可以延长从酵母到无脊椎动物到哺乳动物的各种生物物种的寿命，并且有人认为饮食限制的抗衰老作用是通过减少氧化应激/损伤而发生的。使用Sod 1 −/−小鼠，先前已被证明具有与较短寿命和高肿瘤发生率相关的氧化应激水平增加，我们能够直接测试饮食限制逆转由于氧化应激/损伤增加而导致的衰老表型的能力。我们发现，饮食限制使Sod 1 −/−小鼠的寿命延长了30%，使其恢复到野生型对照小鼠的寿命。通过饮食限制，Sod 1 −/−小鼠的氧化损伤显著降低，如肝脏和大脑F2-异前列腺素（脂质过氧化的标志物）减少所示。对生命末期病理学的分析表明，与随意喂养的Sod 1 −/−小鼠相比，饮食限制显著降低了喂食饮食限制饮食的Sod 1 −/−小鼠的病理学病变的总体发生率，包括淋巴瘤的发生率（27 vs 5%）和总体肝脏病理学。除了总体和肝脏特异性病理学的发生率降低外，在喂食饮食限制饮食的Sod 1 −/−小鼠中，肿瘤性和非肿瘤性病变的负担和严重程度也显著降低。这些数据表明，饮食限制可以显着减弱在Sod 1 −/−小鼠中观察到的由氧化应激/损伤增加引起的加速衰老表型。
Dietary restriction is a powerful aging intervention that extends the life span of diverse biological species ranging from yeast to invertebrates to mammals, and it has been argued that the anti-aging action of dietary restriction occurs through reduced oxidative stress/damage. Using Sod1−/− mice, which have previously been shown to have increased levels of oxidative stress associated with a shorter life span and a high incidence of neoplasia, we were able to test directly the ability of dietary restriction to reverse an aging phenotype due to increased oxidative stress/damage. We found that dietary restriction increased the life span of Sod1−/− mice 30%, returning it to that of wild type, control mice fed ad libitum. Oxidative damage in Sod1−/− mice was markedly reduced by dietary restriction, as indicated by a reduction in liver and brain F2-isoprostanes, a marker of lipid peroxidation. Analysis of end of life pathology showed that dietary restriction significantly reduced the overall incidence of pathological lesions in the Sod1−/− mice fed the dietary restricted-diet compared to Sod1−/− mice fed ad libitum, including the incidence of lymphoma (27 vs 5%) and overall liver pathology. In addition to reduced incidence of overall and liver specific pathology, the burden and severity of both neoplastic and non-neoplastic lesions was also significantly reduced in the Sod1−/− mice fed the dietary restricted-diet. These data demonstrate that dietary restriction can significantly attenuate the accelerated aging phenotype observed in Sod1−/− mice that arises from increased oxidative stress/damage.
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DOI：
10.1126/science.1173635
发表时间：
2009-07-10
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Colman RJ;Anderson RM;Johnson SC;Kastman EK;Kosmatka KJ;Beasley TM;Allison DB;Cruzen C;Simmons HA;Kemnitz JW;Weindruch R
通讯作者：
Weindruch R
影响因子：
4.6
作者：
Han, Eun-Soo;Muller, Florian L.;Richardson, Arlan
通讯作者：
Richardson, Arlan
影响因子：
2.5
作者：
Fontana, Luigi
通讯作者：
Fontana, Luigi
影响因子：
4.8
作者：
Jang, Youngmok C.;Lustgarten, Michael S.;Van Remmen, Holly
通讯作者：
Van Remmen, Holly
DOI：
10.1093/gerona/62.10.1059
发表时间：
2007-10-01
影响因子：
5.1
作者：
McCarter, Roger;Mejia, Walter;Strong, Randy
通讯作者：
Strong, Randy