A cotton annexin protein AnxGb6 regulates fiber elongation through its interaction with actin 1.
A cotton annexin protein AnxGb6 regulates fiber elongation through its interaction with actin 1.
复制标题
棉花膜联蛋白 AnxGb6 通过与肌动蛋白 1 相互作用调节纤维伸长
DOI：
10.1371/journal.pone.0066160
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zuo K
中科院分区：
文献类型：
--
作者：
Huang Y;Wang J;Zhang L;Zuo K
膜联蛋白被认为参与调节棉纤维伸长，但直接证据仍有待提出。本研究克隆了6个在海岛棉纤维中大量表达的膜联蛋白基因（AnxGb）。qRT-PCR结果显示，6个巴氏棉纤维膜联蛋白基因在棉纤维中均有表达，只有AnxGb6基因的表达是棉纤维特异性的。酵母双杂交和BiFC分析表明，AnxGb6同型二聚体与棉纤维特异性肌动蛋白GbAct1相互作用。异位表达的AnxGb6在不增加根细胞数量的情况下提高了拟南芥的根伸长。异位表达AnxGb6导致根细胞伸长区基部积累更多的F-actin。对3种不同纤维长度棉花基因型中AnxGb6的表达分析证实，AnxGb6的表达与棉花纤维长度，尤其是纤维伸长率相关。我们的研究结果表明，AnxGb6可能为F-actin组织提供一个结构域，对纤维伸长很重要。
Annexins are assumed to be involved in regulating cotton fiber elongation, but direct evidence remains to be presented. Here we cloned six Annexin genes (AnxGb) abundantly expressed in fiber from sea-island cotton (G. barbadense). qRT-PCR results indicated that all six G. barbadense annexin genes were expressed in elongating cotton fibers, while only the expression of AnxGb6 was cotton fiber-specific. Yeast two hybridization and BiFC analysis revealed that AnxGb6 homodimer interacted with a cotton fiber specific actin GbAct1. Ectopic-expressed AnxGb6 in Arabidopsis enhanced its root elongation without increasing the root cell number. Ectopic AnxGb6 expression resulted in more F-actin accumulation in the basal part of the root cell elongation zone. Analysis of AnxGb6 expression in three cotton genotypes with different fiber length confirmed that AnxGb6 expression was correlated to cotton fiber length, especially fiber elongation rate. Our results demonstrated that AnxGb6 was important for fiber elongation by potentially providing a domain for F-actin organization.
登录
查看更多内容
影响因子：
5.2
作者：
Gao, Peng;Zhao, Pi-Ming;Xia, Gui-Xian
通讯作者：
Xia, Gui-Xian
DOI：
10.1046/j.1432-1033.2003.03612.x
发表时间：
2003-06-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Hofmann, A;Delmer, DP;Wlodawer, A
通讯作者：
Wlodawer, A
影响因子：
6.2
作者：
Huang, Quan-Sheng;Wang, Hai-Yun;Xia, Gui-Xian
通讯作者：
Xia, Gui-Xian
影响因子：
7.4
作者：
HASENFRATZ, MP;TSOU, CL;WILKINS, TA
通讯作者：
WILKINS, TA
影响因子：
5.1
作者：
Divya, Kesanakurti;Jami, S. K.;Kirti, P. B.
通讯作者：
Kirti, P. B.